摘要
目的:检测和分析白细胞介素-1β(IL-1β)在大鼠慢性牙周炎(CP)和慢性肾衰竭(CRF)模型中的表达。方法:40只SD大鼠随机分为4组,每组10只,正常对照组,慢性牙周炎组,慢性肾衰竭组,牙周炎+慢性肾衰竭(复合组),各组接受相应的建模干预处理。酶联免疫吸附法(ELISA)检测血清IL-1β水平,免疫组化法观察各组肾组织中IL-1β的表达程度,检测肾功能及牙周指标。结果:复合组血清IL-1β水平及肾组织中IL-1β的表达较其他组高(P<0.05,P<0.01),慢性肾衰竭组、复合组血肌酐(Scr)、尿素氮(BUN)高于对照组、慢性牙周炎组(P<0.05)。慢性牙周炎组、复合组牙龈附着丧失(AL)高于对照组、慢性肾衰竭组(P<0.05)。结论:CP与CRF之间可能具有相关性,IL-1β的表达可能与两疾病的发生发展有关。
Objective：To detect and analyse IL-1β level in rat model of chronic periodontitis （CP） and chronic renal failure（CRF）.Methods：Forty SD rats were randomly and equally divided into four groups：the control group,the CP group,the CRF group,and the group of CP and CRF.Every group received the corresponding treatment.IL-1β level in serum was assayed by ELISA.IL-1β in nephridial tissue was observed with immunohistochemisty.Renal function and periodontal target were detected.Results：Serum and nephridial tissue levels of IL-1β in the group of CP and CRF were higher than those of other groups （P0.05,P0.01）.Serum levels of Scr and BUN in group CRF,CP and CRF group were higher than those in the control group and the CP group （P0.05）.Attachment loss levels in the CP group and the group of CP and CRF were higher than that of the control group and the CRF group （P0.05）.Conclusion：There might possibly be correlation between chronic periodontitis and chronic renal failure,and the development of the two diseases might be correlated with the expression of IL-1β.
出处
《中国中西医结合肾病杂志》
2010年第7期586-588,I0001,共4页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
新疆维吾尔自治区重点学科(内科学)建设项目(No.2004-2008)
