氧化酚砷诱导急性早幼粒细胞性白血病细胞凋亡的研究

Phenylarsine oxide induces apoptosis in acute promyelocytic leukemia cell line NB_4

目的：了解氧化酚砷（phenylarsineoxide,PAO）对急性早幼粒细胞性白血病（APL）细胞系NB4细胞的可能作用。方法：在台盼蓝排除法计数活细胞和细胞活力的基础上，通过细胞形态学和流式细胞仪检测细胞凋亡。通过对细胞进行Rhodamine（Rh）123和碘化丙啶双重染色，并应用流式细胞仪检测其荧光强度，以反映细胞线粒体跨膜电位（△Ψm）。结果和结论：低浓度（005和0．1μmol／L）的PAO明显抑制NB4细胞的生长，并显著降低其细胞活力。进一步地，细胞形态学观察和流式细胞仪分析显示，这些浓度的PAO诱导NB4细胞凋亡。该效应可能与△Ψm下降密切相关。

Purpose:To understand the possible effects of phenylarsine oxide (PAO) on acute promyelocytic leukemiacell line NB4.Methods:Viable cell numbers and viahility were counted by means of trypan-blue exclusion.Cell apoptosiswas assessed through morPhology and cytometry.The mitochondrial transmembrame potentials(△Ψm) were detected throughdouble staining of Rhodamine123 and propidium iodide,of which fluorescent intensities were measured on flow cytometry.Results and Conclusion:Low concentrations(0.05 and 0.1μmol/L) of PAO significantly inhibited cell growth and decreased viability in NB4 cells.Furthermore,cytomorphology and flow cytometry showed that these concentrations of PAO induced NB4 cell apoptosis, which could associate closely with the △Ψm collapse induced by PAO.