中国南方鼻咽鳞状细胞癌与EB病毒感染的关系(英文)

Association of Epstein-Barr Virus With Nasopharyngeal Squamous Cell Carcinoma in the South of China

目的：探讨中国南方鼻咽癌高发区鼻咽鳞状细胞癌或称角化性鳞状细胞癌（Keratinizingsquamouscellcarcinoma，KSCC）是否也如非角化性癌那样与EB病毒感染有密切的关系。方法：应用PCRSouthernblotting、原位分子杂交和免疫组化法检测38例鼻咽鳞状细胞癌组织中EB病毒DNA及其产物EBNA－1，EBNA－2，EBERs，LMP-1，ZEBRA，EA－D，VCA和MA的表达。结果：（1）37例鳞状细胞癌组织中有EBERs阳性癌细胞出现，阳性率达97．4％（37／38）；（2）在37例鳞状细胞癌中的大部分未分化和低分化癌细胞表达EBNA－1和EBERS，21例（56．8％，21／37）组织内有LMP－1表达；（3）其中23例（62．2％，23／37）组织中只有少数癌细胞表达ZEBRA或／和EA－D；（4）22例（59．5％，22／37）组织中分化较好的或角化性癌细胞表达VCA或／和MA。结论：（1）中国南方地区鼻咽鳞状细胞癌与非角化性癌一样与EB病毒的感染有着较恒定而非偶然的关系；（2）鳞状细胞癌组织中大多数未分化和低分化癌细胞均以11型潜伏状态感染EB病毒，主要表达EBNA－1、EBERS和LMP－1，其中一小部分癌细胞也表达溶解期流产型蛋白ZEBRA和EA－D；（3）EB病毒在大部分分化好的和角化性癌细胞中则能够完成其复制周期并表达溶解晚期蛋白VCA或／和MA。

Objective:To investigate whether the nasopharyngeal squamous cell carcinoma or keratinizing squamous cell carcinoma(KSCC) is also closely associated with Epstein-Barrr virus infection as nasopharyngeal non-keratinizing carcinoma does. Methods:The Epstein-Barr virus (EBV) DNA and its gene expression products,including EBNA-1,EBNA-2, EBERs,LMP-1, ZEBRA, EA-D, VCA and MA were detected in 38 nasopharyngeal KSCCs using PCR Southernblotting hybridization, in situ hybridization and immunohistochemistry. ResultS: (1) The EBERs Positive cancer cells could be found in 37 KSCCs (97. 4%, 37/38). (2) Most undifferentiated and poorly differentiated cancer cells of these 37 KSCCsexpressed EBNA-1 and EBERs; and LMP-1 positive cells could be found in 21 specimens (56. 8%, 21/37). (3) A few ofZEBRA positive or/and EA-D positive cancer cells were found in 23 out of 37 KSCCs (62. 2%, 23/37). (4) Several ofmore differentiated or keratinizing cancer cells expressed VCA or/and MA in 22 of 37 KSCCs (59. 5%, 22/37). Conclusions: (1) KSCCs of the nasopharynx in the south of China are also consistently, not occasionally, associated with EBV infection as non-keratinizing carcinomas (NKCs) do; (2) The majority of undifferentiated and poorly differentiated cancer cells in KSCCs are always infected with EBV in type II latency, mainly expressing EBNA-1, EBERs, and LMP-1,and a small number of them may express the abortive immediate early and early viral proteins (ZEBRA & EA-D); (3) The more differentiated or keratinizing cancer cells infected with EBV in KSCCs might complete its production cycle and express late viral proteins (VCA or/and MA).