喉粘膜上皮异型增生p53、H-ras过度表达与预后的关系

The relationship between the overexpression of p53, H-ras and the prognosis of laryngeal dysplasia

目的：探讨喉粘膜上皮异型增生p53、H－ras过度表达与预后的关系。方法：应用免疫组化ABC法对63例喉粘膜上皮异型增生（经随访其中40例恶变为癌）、56例喉鳞癌和7例喉正常组织进行检测。结果：p53蛋白阳忖率在喉粘膜卜皮异型增生恶变组为50．0％，未恶变组为17.4％，喉癌组为51.8％，正常组织无表达。恶变组与未恶变组间，未恶变组与喉癌组间的p53蛋白表达差异有统计学意义（分别为P＜0．05和p＜0.01）。H－ras蛋白阳性率在喉正常组织、异型增生恶变组、未恶变组、喉癌组依次为0％、52．5％、522％和73．2％。恶变组和未恶变组间H－ras表达差异无统计学意义。结论：p53蛋白的过度表达与喉粘膜上皮异型增生的预后有密切关系。H－ras的表达与喉粘膜上皮异型增生的预后无关。

Purpose: In order to determine whether prognosis of laryngeal dysplasia is related to expression of p53,H-ras. Method: The avidin biotin peroxidase method was used to detect the p53, H-ras protein in 63 cases of dysplasia (40 cases of whom developed into cancer after 4 years follow-up.), 56 squasmous cell carcinoma and 7 normal tissues. All samples were from larynx. Results:The results showed that the positive p53 staining rates was 50% in the malignant change group, 17. 4% in the nonmalignant group. and 51. 8% in cancer. No protein was found in normal tissues. The statistical significance was positive between the malignant change group and the nonmalignant group. The positive H-ras staining rates were 0%, 52. 5%, 52. 2% and 73. 2% in nomal tissues, malignant change group, nonmalignant one and cancer, respectivly. There was no statistical difference between the malignant change group of dysplasia and the nonmalignant one. Conclusion: The study results indicate that the overexpression of p53 protein was closely related to the prognosis of laryngeal dysplasia.