摘要
目的研究临床分离的肺炎克雷伯菌对头孢噻肟的耐药性与超广谱β内酰胺酶（ＥＳＢＬ）及其与感染性疾病的关系。方法应用纸片扩散法检测肺炎克雷伯菌对头孢噻肟的耐药性，应用双纸片协同试验检测ＥＳＢＬ；脉冲凝胶电泳（ＰＦＧＥ）对细菌分型。结果肺炎克雷伯菌对头孢噻肟５６株中介、６２株耐药，双纸片协同试验阳性为９５株，阳性率８１％。应用ＰＦＧＥ法将８８株被测菌株分为２８个不同的分型，其中有些分型中含有多个亚型。结论对头孢氨噻肟中介及耐药的肺炎克雷伯菌多数产生ＥＳＢＬ。ＰＦＧＥ对肺炎克雷伯菌感染流行病学监测是一个有效的方法。
Objectives To study the prevalence of cefotaxime resistance and extended spectrum β lactamase in Klebsiella pneumoniae, and to dertermine the existence of hospital infection by this organism.Methods Cefotaxime resistance was tested by NCCLS disk diffusion method. The organisms ware tested for ESBL production by double disk synergy and the patterns of XbaI digested chromosomal DNA of ESBL producing K. Pneumoniae isolates were analyzed by pusled field gel electrophoresis.Results Among the strains with intermediate or resistance to cefotaxime, 81% were double disk synergy test positive. ESBL producing K. Pneumoniae were grouped into 28 chromosomal DNA patterns by XbaI digestion.Conclusions PFGE was useful in epidemiological monitoring etiology of infectious disease.
