沙苑子提取物中多酚含量的测定

Determination of Polyphenols in Different Organic Solvent Extracts of Semen Astragali complanati

研究Folin-Ciocalteu比色法测定沙苑子中多酚含量的优化条件,并以优化的条件测定沙苑子不同溶剂提取物中多酚的含量。结果表明:Folin-Ciocalteu显色剂(经稀释10倍)用量1.00mL、7.5g/100mLNa2CO3用量3.00mL、反应温度25℃、反应时间120min、测定波长647nm为沙苑子中多酚含量测定的最佳条件,所用没食子酸标准品在10～100μg/mL范围内与吸光度呈良好的线性关系(y=0.0081x-0.0249,R2=0.9996);沙苑子甲醇、乙醇和丙酮溶液提取物的质量浓度为1g/100mL左右时较适合测定其多酚的含量。

In order to provide an optimal Folin-Ciocalteu assay for determining polyphenols in different organic solvent （ethanol aqueous solution,acetone aqueous solution and methanol aqueous solution all at 60% cocentration） extracts of Semen Astragali complanati,some Folin-Ciocalteu assay conditions were optimized.The results indicated that the optimal conditions for the determination of polyphenols by Folin-Ciocalteu were as follows： 10-fold diluted Folin-Ciocalteu reagent amount 1.00 mL,7.5 g/100 mL Na2CO3 amount 3 mL,reaction temperature 25 ℃,reaction time 120 min,and determination wavelength 647 nm.A good linear relationship was observed between absorbance and gallic acid concentration within the range of 10-100 μg/ mL （y = 0.0081x -0.0249,R2 = 0.9996）.In addition,accurate contents of polyphenols in methanol extract,ethanol extract and acetone extract of Semen Astragali complanati diluted to a concentration of 1 g/100 mL was determined by this method.