PSE-HPLC法测定食品中叔丁基羟基茴香醚和2,6-二叔丁基羟基甲苯

Pressurized Solvent Extraction and HPLC Determination of BHA and BHT in Food

目的:建立测定食品中抗氧化剂叔丁基羟基茴香醚(BHA)和2,6-二叔丁基羟基甲苯(BHT)加压溶剂萃取-高效液相色谱法(PSE-HPLC法)。方法:采用正交试验对影响PSE萃取效率的温度、压力、萃取溶剂、萃取时间进行优化,联合HPLC进行测定,并确定分别以BHA提取量、BHT提取量以及总量为评价指标的最优条件。结果:PSE-HPLC法测定BHT和BHA的相对标准偏差(RSD)为0.5%～3.1%,并且在1.0～200.0μg/mL范围内色谱峰面积与组分质量浓度均有很好的线性相关性(r≥0.9997),检出限为0.05μg/mL,在最优条件下的回收率为92.60%～97.80%。结论:PSE-HPLC法简便、快速、效率高,方法的重现性、线性相关性以及检出限理想,适用于食品中BHT和BHA含量及两者总量的同时快速检测。

The purpose of this study was to establish a method for determining butylated hydroxyanisole （BHA） and butylated hydroxytoluene （BHT） in food using pressurized solvent extraction （PSE） and HPLC.The simultaneous PSE extraction of BHA and BHT was optimized using orthogonal array design with respect to the effects of extraction temperature and pressure,extraction solution type and length of extraction,and the optimum values of these extraction parameters for achieving the maximum sum of extraction yields of BHA and BHT were.Under the optimized PSE extraction conditions,the average spike recoveries for BHA and BHT determined by the analytical method ranged from 92.60% to 97.80% with RSDs from 0.5% to 3.1%.The developed method exhibited good linearity over the range from 1.0 to 200.0μg/mL,with a correlation coefficient of no less than 0.9997 and the detection limit was 0.05μg/mL This method is simple,fast and efficient and has good repeatability.It is most suitable for the simultaneous determination of BHA and BHT in food.