急性冠状动脉综合征患者外周Th17细胞水平检测及临床意义

Detection of Th17 cells in peripheral blood of patients with acute coronary syndrome and its significance

目的探讨急性冠状动脉综合征(ACS)患者外周血中辅助性T细胞17(Th17)的异常与疾病的关系。方法采用流式细胞术检测18例胸痛综合征(CPS)、17例稳定性心绞痛(SA)、21例不稳定性心绞痛(UA)、20例急性心肌梗死(AMI)患者的外周血Th17细胞水平。应用酶联免疫吸附方法检测其外周血Th17相关细胞因子白细胞介素(IL)-17的表达。结果 AMI组和UA组外周血CD4+IL17+细胞占CD4+T细胞比例分别为(4.04±0.93)%、(3.07±0.50)%,明显高于SA组的(1.45±0.35)%和CPS组的(1.04±0.24)%(P<0.01),SA组的CD4+IL-17+细胞占CD4+T细胞比例较CPS组也有明显增加。AMI组和UA组IL-17水平分别为(55.91±5.13)pg/ml、(46.65±8.72)pg/ml,较SA组(17.13±4.13)pg/ml和CPS组(12.28±2.03)pg/ml明显增加。结论 ACS患者外周血Th17细胞水平增加,IL-17水平相应增加,Th17细胞可能参与动脉粥样硬化斑块不稳定和ACS的发病。

Objective To investigate the changes of T help 17（Th17） cells in peripheral blood of patients with acute coronary syndrome （ACS） and to identify its relationship with ACS.Methods 18 cases chest pain syndrome（CPS） arteries,17 patients with stable angina（SA）,21 patients with unstable angina（UA）, and 20 patients with acute myocardial infarction（AMI） were studied.The frequencies of Th17 in peripheral blood were measured by flow cytometry.The serum levels of Th17-related cytokines （IL-17） were determined by enzyme linked immunosorbent assay （ELISA）.Results The ratios of CD4^＋IL17^＋/CD4＋ T cells in ACS patients were significantly higher than those in the stable angina patients and CPS, while there was also obvious difference between the SA and CPS. Levels of IL-17 in patients with AMI and UA were increased significantly compared with patients with SA and CPS.Conclusion The levels of Th17 and IL-17 increase obviously in patients with ACS.Th17 may play a potential role in the progression and stability of atheroma.