星星草脱氢抗坏血酸还原酶(PtDHAR)cDNA的克隆及表达分析

Cloning and Expression Analysis of Dehydroascorbate Reductase Gene (PtDHAR) in Puccinellia tenuiflora (Griseb.) Scribn.et Merr

脱氢抗坏血酸还原酶是抗坏血酸代谢循环中的关键酶,在多种植物中与抗胁迫相关。为了获得抗盐碱植物星星草中该基因序列,利用RACE技术,从星星草中克隆出脱氢抗坏血酸还原酶基因(PtDHAR)的cDNA全长序列,其GenBank登录号为HM125046。PtDHAR cDNA核苷酸序列长度为987bp,开放阅读框为639bp,编码213个氨基酸。该基因编码的氨基酸序列与水稻、小麦等禾本科作物具有很高的同源性。Northern杂交分析表明,该基因在盐碱胁迫下表达量显著升高。

Dehydroascorbate reductase （DHAR） is the key enzyme in ascorbate metabolic cycle which was reported related with stress resistant in many plants.RACE technology was employed to obtain full-length cDNA from Puccinellia tenuiflora named PtDHAR （GenBank accession number HM125046）,which was 987 bp length.The open reading frame encoding 213 amino acids which had a high identity with DHAR from Oryza sativa and Triticum aestivum.Northern blotting analysis showed that the expression level of PtDHAR under saline-alkali stress was significantly up-regulated than that with no stress.