胶质瘤性脑水肿中AQP4与VEGF的关系研究

Relationship between Aquaporin-4 and Vascular Endothelial Growth Factor in Cerebral Edema Induced by Gliomas

目的探讨水通道蛋白4(AQP4)在脑胶质瘤性脑水肿的分子调节机制及与血管内皮生长因子(VEGF)的关系。方法收集2007年10月2008年6月间65例脑胶质瘤患者手术切除新鲜肿瘤标本(胶质瘤Ⅰ级6例、Ⅱ级18例、Ⅲ级11例、Ⅳ级30例)。应用免疫荧光细胞化学方法检测肿瘤组织中AQP4蛋白和VEGF蛋白的阳性表达情况,并分析AQP4和VEGF的表达差异与关系。结果免疫荧光细胞化学法染色显示,AQP4蛋白在正常脑组织中主要表达于细胞膜表面,胞浆和细胞核着色较浅。在胶质瘤细胞内,AQP4广泛分布于胞浆内;肿瘤中AQP4表达和VEGF呈正相关(r=0.877,P=0.000)。结论在胶质瘤性脑水肿中,AQP4在胶质瘤细胞内主要分布于胞浆内,且与VEGF呈明显正相关。

Objective To study the molecular mechanisms of aquaporin-4 （AQP4） in cerebral edema,and the relationshoip between AQP4 and vascular endothelial growth factor （VEGF）. Methods The tissues of glioma,a total of 65 patients （WHO grade Ⅰ in 6 cases,grade Ⅱ in 18 cases,grade Ⅲ in 11 cases,and grade Ⅳ in 30 cases）,were obtained from those who received the operation in the Department of Neurosurgery of West China Hospital from October 2007 to June 2008. The immunofluorescence cytochemistry method was used to detect the positive expression of AQP4 protein and VEGF protein in gliomas. Then it was analyzed on the relationship between AQP4 protein and VEGF protein. Results Immunofluorescence cytochemistry staining of the slices showed that AQP4 protein mainly expressed in the cellular membrane of the normal brain tissue,and slightly expressed in cytoplasm and caryon,but in glioplastic cell,AQP4 protein mainly expressed in the cytoplasm. The expression of AQP4 in tumor had positive correlation with VEGF （r= 0.877,P=0.000）. Conclusions In gliomas,especially in high grade gliomas,AQP4 protein mainly express in the cytoplasm,and the expression of AQP4 positively correlate with VEGF.