重型颅脑损伤患者脑挫伤组织水通道蛋白-4的表达及意义

Expression of aquaporin-4 in the brain tissues from patients with severe brain injuries and its significance

目的 研究重型颅脑交通伤患者脑挫伤组织核心区、边缘区水孔蛋白质-4（AQP-4）的表达水平及其意义.方法 选取2007年1月-2009年7月我科重型颅脑交通伤手术患者（额颞区脑挫伤）30例,按受伤至手术时间长短平均分为O～4 h组（A组）、4～8 h组（B组）和8～12 h组（C组）.每位患者术中分别取挫伤组织边缘区及核心区组织各一.以桥小脑区肿瘤切除术中为获取手术通道而切除的5例患者正常脑组织并分成10份作为对照,应用Western-blot方法,以GAPDH为内参,分别检测边缘区、核心区挫伤组织及正常脑组织中AQP-4的表达水平.结果 边缘区组织AQP-4表达随时间增加而逐步升高,也明显高于核心区和正常组的表达水平,而核心区组织中AQP-4的表达低于正常脑组织.结论 边缘区AQP-4的高表达在伤后早期即开始,且有明显的时间依赖性.而核心区AQP-4的表达水平较低可能与血脑屏障及其周围结构的早期破坏有关.

Objective To study the expression of aquaporin-4 （AQP-4） in core and marginal region of the contusion brain tissues from patients with severe traffic brain injuries. Methods Thirty patients with severe traffic brain injuries （frontal-temporal brain contusion） admitted into our department from January 2007 to July 2009 were enrolled in the study and divided into three groups according to the period from injury to operation, ie, 0-4 hours （Group A）, 5-8 hours （Group B） and 9-12 hours （Group C）. The tissue was collected from core and marginal regions of brain contusion in each group. Ten parts of normal brain tissues obtained from the cerebellum to gain good exposure of CPA region tumors were used as control. The expression of AQP-4 in the normal brain tissues and in the tissues from core and marginal region of brain contusion, with GAPDH used as a control. Results The expression of AQP-4 in the marginal region was distinctly higher than that in normal tissues and in the tissues from core region. The AQP-4 expression in the tissues of the core region was lower than that in normal tissues. Conclusions AQP-4 is highly expressed in the tissues from the marginal region early after injury in a time-dependent fashion. Low expression level of AQP-4 in the core region is possibly correlated with early damage of blood brain barrier and peripheral structures.