摘要
目的:采用HPLC柱前程序衍生法测定复方α-酮酸片中5种氨基酸的含量。方法:以辛烷基硅烷键合硅胶为填充剂,梯度洗脱方式,柱温为40℃,以邻苯二甲醛为衍生试剂,检测波长为338 nm。检测复方α-酮酸片中L-醋酸赖氨酸、L-苏氨酸、L-色氨酸、L-组氨酸、L-酪氨酸的含量。结果:L-醋酸赖氨酸、L-苏氨酸、L-色氨酸、L-组氨酸和L-酪氨酸检测浓度的线性范围分别为0.10~5.25(r=0.999 9)、0.052~2.65(r=0.999 8)、0.022~1.15(r=0.999 9)、0.038~1.91(r=0.999 7)、0.030~1.51(r=1.000 0)mg·mL-1;平均回收率为99.8%~100.1%(RSD均为0.1%)。结论:该方法灵敏度高,专属性强,结果准确可靠,可用于测定复方α-酮酸片中氨基酸的含量。
OBJECTIVE: To establish an HPLC method with pre-column programming derivatization for the content determination of 5 kinds of amino acids in Compound α-ketoacid tablets.METHODS: The separation was performed on C8 column(gradient elution) with column temperature at 40 ℃.OPA was used as the derivation reagent.The detection wavelength was set at 338 nm.The contents of L-lysine acetate,L-threoine,L-tryptophan,L-histidine,L-tyrosine in Compound α-ketoacid tablets were determined.RESULTS: The linear ranges of L-lysine acetate,L-threoine,L-tryptophan,L-histidine,L-tyrosine were 0.10~5.25 mg·mL^-1(r=0.999 9),0.052~2.65 mg·mL^-1(r=0.999 8),0.022~1.15 mg·mL^-1(r=0.999 9),0.038~1.91 mg·mL^-1(r=0.999 7),0.030~1.51 mg·mL^-1(r=1.000 0),respectively.The average recovery was 99.8%~100.1%(RSD=0.1%).CONCLUSION: This method is exclusive,sensitive and reliable for the content determination of amino acids in Compound α-ketoacid tablets.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第29期2768-2770,共3页
China Pharmacy
关键词
HPLC法
程序衍生化
复方Α-酮酸片
氨基酸
含量
HPLC
Programming derivatization
Compound α-ketoacid tablets
Amino acids
Content