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TGF-β1诱导脐带血CD4＋CD25＋调节性T细胞Foxp3和IL-10表达 被引量：5

Role of TGF-β1 in the expression of Foxp3 and IL-10 on CD4^+CD25^(high) Treg in neonatal cord blood
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摘要 为观察经转化生长因子β1(TGF-β1)诱导的新生儿脐带血Treg中Foxp3和IL-10表达特点,探讨TGF-β1在诱导产生的新生儿脐带血Treg中的作用。将脐带血单个核细胞用CD3mAb激活后,加IL-2培养扩增,同时加入不同浓度的TGF-β1干预,扩增第4天分别用流式细胞术和RT-PCR技术检测CD4+CD25+TregFoxp3蛋白和Foxp3 mRNA的表达,用ELISA法检测扩增细胞培养上清液中IL-10水平。结果显示,TGF-β1诱导下脐带血CD4+CD25+TregFoxp3蛋白和Foxp3 mRNA的表达明显增加,培养上清中IL-10含量也明显增加。表明,TGF-β1在体外能够促进脐带血CD4+CD25+Treg的Foxp3的表达和IL-10分泌。 To observe the expression of Foxp3 and IL-10 on CD4＋CD25＋ Treg induced by transforming growth factor-β1 （TGF-β1） in neonatal cord blood,cord blood mononuclear cells（CBMC） were stimulated with CD3 mAb and cultured in IL-2 containing medium with different concentrations of TGF-β1. The expressions of Foxp3 protein and Foxp3 gene mRNA in CD4＋CD25＋ Treg were detected by flow cytometry and RT-PCR assay,respectively,and the level of IL-10 in supernatant from cultured CBMC was detected by enzyme linked immunosorbent assay （ELISA）. It was demonstrated that the expression of Foxp3 protein and mRNA in CD4＋CD25＋ Treg and IL-10 level in supernatant of cultured CBMC increased significantly in TGF-β1 treated group compared to control group. It is concluded that TGF-β1 up-regulates Foxp3 expression of the CD4＋CD25＋Treg and increases the secretion of IL-10 in cord blood in vitro.
作者 唐洁 梁娟 李柏青
机构地区 蚌埠医学院免疫学教研室 省感染与免疫重点实验室蚌埠医学院 蚌埠市第三人民医院妇产科
出处 《现代免疫学》 CAS CSCD 北大核心 2010年第4期313-317,共5页 Current Immunology
基金 安徽省省高校省级重点实验室重点科研项目(2004sys008) 蚌埠医学院科研项目(BY0705)
关键词 调节性T细胞 新生儿脐带血 TGF-Β1 FOXP3 IL-10 regulatory T cell neonate cord blood Foxp3 IL-10
分类号 R392.11 [医药卫生—免疫学]
  • 相关文献

参考文献15

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二级参考文献20

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共引文献9

同被引文献71

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二级引证文献23

现代免疫学
2010年 第4期

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