摘要
目的探讨姜黄素对大鼠脑缺氧缺血损伤时NOS活力、caspase-3表达及细胞凋亡的影响。方法采用大鼠Rice模型。雄性SD大鼠48只，每组6只。随机分为假手术对照组（SH组）、脑缺氧缺血组（HI组）、姜黄素组（CU组）、溶剂对照组（SC组）；生化方法检测脑组织NOS（一氧化氮合酶）活力；免疫组织化学测定大脑海马CA1区caspase-3（半胱氨酸天冬氨酸特异性蛋白酶-3）的表达；电镜观察大脑海马形态学结构变化。结果姜黄素可显著减少大脑海马神经细胞损伤数量，NOS酶活力含量明显减低，并且抑制caspase-3蛋白的表达。结论细胞凋亡参与了大脑缺氧缺血损伤的发生，姜黄素可能通过减低NOS酶活力、下调caspase-3的表达，从而抑制细胞凋亡，减轻脑缺氧缺血性损伤。
Objective To observe the effects of curcumin on apoptosis and caspase-3 expression in hippocampus tissue area in cortex with hypoxic ischemic brain damage. Methods A total of 48 SD rats were randomly divided into four groups as the following： sham group（SH）, hypoxic ischemic brain damage group（HIBD）, curcumin group（CU） and solvent control group（SC）. Brain specimens drawn from the left cerebral cortex were tested for the enzyme activity of NOS. The expression of caspase-3 protein in hippocampus tissue were detected with immunohistochemistry （IHC）, ultrastructural changes of cortex tissue were observed by transmission electron microscopy. Results The The enzyme activity of NOS in CU group was clearly lower than those in HI group at the same reperfused time （P〈0.01）. The expression of caspase-3 was decreased in cortex tissue in CU group than that in IR group, the number of apoptosis neurons in hippocampus tissue were much less in CU group than that in HI group. Conclusion Neuron apoptosis participates in hypoxic ischemic brain damage. The protective effects of curcumin include suppressing the expression of caspase-3 and blocking apoptosis in hypoxic ischemic brain damage.
出处
《实验动物与比较医学》
CAS
2010年第3期158-162,共5页
Laboratory Animal and Comparative Medicine
基金
国家自然基金（30472269）
浙江省教育厅（20030798）
