摘要
目的研究拘束冷应激对大鼠血清中促肾上腺皮质激素（ACTH）、皮质醇（CORT）、肌酸激酶（CK）和丙氨酸转氨酶（ALT）浓度的动态变化及可能的机制。方法50日龄清洁级大鼠56只，随机分为7组，即0、0．5、1．0、1．5、2．0、2．5和3．0h组。采用拘束冷应激法，在应激相应的时间后，检测大鼠血清中ACTH、CORT、CK、ALT浓度和胃溃疡指数的测定。结果①大鼠血清中的ACTH浓度随着应激时间的增加呈现迅速升高的趋势，0．5h达到最高值，之后又迅速下降，逐渐趋于平缓；②血清中的CORT浓度呈现逐渐升高的趋势。1．5h达到最高值，之后又迅速下降，逐渐趋于平缓；③血清中的CK和ALT活力显著升高，且随着冷应激时间的增加而递增；④大鼠胃溃疡指数在应激初期呈现迅速升高的趋势，2．5h时达到最高值。结论拘束冷应激可使大鼠血清中的ACTH和CORT的浓度上升，至最高值后迅速下降；而血清中CK和ALT的浓度则随着拘束冷应激时间的增加而递增。拘束冷应激导致大鼠发生急性胃溃疡，应激2．5h时最严重，提示拘束冷应激大鼠模型的造模时间以2．5h为最佳。
Objective To study the dynamic change of the level of ACTH, CORT, CK and ALT in serum of rats caused by cold restraint stress, and the possible mechanisms. Methods Fifty-six SD rats of 50 days old were divided randomly into 7 groups with testing time of 0 h, 0.5 h, 1.0 h, 1.5 h, 2.0 h, 2.5 h, 3.0 h. After putting each group rats under testing environment by using the cold restraint stress method in specified group testing time, the level of ACTH, CORT, CK, ALT and UI in serum were analyzed. Results （1）The ACTH level in serum rises quickly with time increasing for the rats under cold restraint stress, it reaches the highest value at 0.5 h, and then it starts to fall quickly. （2） The CORT level rises gradually with time increasing, it reaches the highest value at 1.5 h, and then it starts to fall quickly. （3） The CK and ALT levels rise gradually with time increasing for the rats under cold restraint stress. （4） The UI level rises quickly with time increasing, it reaches the highest value at 2.5 h. Conclusion The cold restraint stress can cause rising of the ACTH and CORT level in serum of rat, after it reaches the highest value, it falls back quickly. It can cause CK and ALT to rise with time increasing. Cold restraint stress can cause acute gastric ulcer of rats, with the worst at 2.5 h. It suggests that the best time for creating cold restraint stress model in rat is 2.5 h.
出处
《实验动物与比较医学》
CAS
2010年第3期205-208,共4页
Laboratory Animal and Comparative Medicine
基金
温州市科技计划项目（No：Y20060106）,温州医学院科技发展基金资助项目（No：200801052）
