C14orf166异位表达对Hela细胞增殖与迁移能力的影响

Ectopic expression of C14orf166 and its effect on Hela cell proliferation and immigration

目的:研究C14orf166异位表达对肿瘤细胞增殖和迁移能力的影响。方法:构建C14orf166真核表达载体并转染Hela细胞,采用RT-PCR及蛋白质印迹法技术检测基因表达,用MTT、Transwell的方法检测C14orf166蛋白在Hela细胞的异位表达对其增殖和迁移能力的影响。结果:成功构建pcDNA3.1-C14orf166真核表达载体并使其在Hela细胞中大量表达,初步研究发现重组质粒转染组与空质粒转染组相比,细胞迁移能力增强,细胞穿膜数分别为39.6±11.7和25.7±8.6,而细胞增殖能力并没明显变化。结论:获得了真核表达载体pcDNA3.1-C14orf166,初步证实C14orf166表达能促进Hela细胞的迁移,为进一步研究c14orf166的功能奠定了基础。

OBJECTIVE：To investigate C14orf166 ＇s effect on Hela cell proliferation and immigration by ectopic expression.METHODS：Eukarytic expression vector consisting of C14orf166 gene was constructed,which was transfected into Hela cells.C14orf166 expression was analysed using western blot and RT-PCR.The effect of C14orf166 on Hela cell proliferation and immigration were investigated by MTT assay and Transwell assay.RESULTS：The recombinant eukaryotic expression vestor pcDNA3.1-C14orf166 was successfully constructed and expressed in Hela cells;The number of pcDNA3.1-C14orf166 transfected cells to traverse membrane was obviously increased compared with that of empty vector transfected cells,（39.6±11.7）vs（25.7±8.6）,while in MTT assay,no significant difference was observed between recombinated vetor transfected cells and empty vector transfected cells.CONCLUSION：The eukaryotic expression vector pcDNA3.1-C14orf166 is obtainded;it is verified that C14orf166 can enhance Hela cell immigration,which provide foundation for further reseach.