聚甲基丙烯酸甲酯骨水泥作为抗肿瘤药物缓释载体的实验研究

Basic research of polymethyl methacrylate bone cement as drug delivery carrier

目的:探讨聚甲基丙烯酸甲酯(PMMA)骨水泥加入抗肿瘤药物后物理和力学性能的变化,以及抗肿瘤药物在体外释放的情况。方法:在40 g骨水泥中分别加入50 mg多柔比星、20 mg唑来膦酸,并设立单纯的骨水泥组作为对照。在无菌条件下将骨水泥按照ISO5833:2002《外科植入物——丙烯酸类树脂骨水泥》的标准制成各种试件,测定其抗压强度、抗弯模量和抗弯强度。同时将试件进行浸泡洗提,检测其药物的释放情况。结果:与对照组相比,骨水泥的物理和力学性能没有明显的改变,对照组和实验组骨水泥的抗压强度、抗弯模量、抗弯强度,差异无统计学意义,P>0.05。两种药物均能从骨水泥中缓慢的释放出来,药物的释放规律及释放量基本相同,多柔比星释放总量占药物初始剂量的0.83%,唑来膦酸释放总量占药物初始剂量的0.66%。结论:抗肿瘤药物能有效地从骨水泥中缓慢的释放,40 g骨水泥中加入<1 g的药物不会影响骨水泥的物理和力学性能,PMMA骨水泥适合作为抗肿瘤药物的缓释载体。

OBJECTIVE： To investigate the influence of the physical and mechanical properties of polymethyl methacrylate（PMMA） bone cement mixed with anti-cancer drugs,and the situation of the release of the drugs in vitro.METHODS： Forty g PMMA was mixed with 50 mg doxorubicin hydrochloride and 20 mg zoledronic acid respectively which were divided into GroupⅡ and Group Ⅲ,and the PMMA mixed with nothing was GroupⅠ.In accordance with the ISO5833： 2002 ＂surgical implants-acrylic resin cement＂,a variety of bone cement samples were made in aseptic conditions.The compressive strength,flexural modulus and flexural strength of the samples were checked,the release situation of the drugs was checked after soak elution of the samples.RESULTS： The compressive strength,flexural modulus and flexural strength of bone cement were not significant different between experimental groups and control group（P〉0.05）.The drugs can be released from the bone cement,and the release law and quantity were not significantly different between groupⅡ and group Ⅲ.Throughout the elution process,more than 0.83% of the doxorubicin hydrochloride and 0.66% of zoledronic acid were released.CONCLUSIONS： There is no influence on the physical and mechanical properties of bone cement when 40 g of which is mixed with less than 1g of the drug.The anti-cancer drugs can be released from bone cement effectively.The PMMA bone cement can be a release carrier of anti-cancer drugs.