摘要
目的:探讨二烯丙基二硫(DADS)对白血病K562细胞增殖的影响,以及Bcl-2表达的调节作用。方法:用DADS预处理白血病K562细胞构建细胞模型,MTT法分别检测不同DADS浓度(5gmL-1、10gmL-1、20gmL-1、40gmL-1)和不同处理时间(6h、12h、24h、48h)细胞增殖情况,IC50浓度处理白血病K562细胞之后,Westernblot和RT-PCR检测Bcl-2蛋白和mRNA表达水平。结果:MTT结果显示DADS能够抑制白血病K562细胞的增殖,且呈剂量和时间依赖性;IC50浓度的DADS处理K562细胞24小时后,Bcl-2蛋白和mRNA的表达量显著减少。结论:DADS可显著抑制K562细胞增殖,而这一作用可能与Bcl-2表达下调有关。
Objective:To explore the effect of diallyl disulfide(DADS) on proliferation of K562 cell and expression of Bcl-2.Methods:Leukaemia K562 cells line treated with DADS(5 g mL^-1,10 g mL^-1,20 g mL^-1,40 g mL^-1) and time(6h,12h,24h,48h),and proliferation of cells were examined by MTT,Bcl-2 protein and transcriptional level was measured by Western blotting and RT-PCR after treated with DADS at concentration of IC50.Results:MTT assay showed that DADS significantly inhibited proliferation of K562 cells in a time-and concentration-dependent manner.The protein and mRNA expression of Bcl-2 were significantly decreased after treated with DADS at the IC50 for 24 hours.Conclusions:Significant inhibition of DADS in proliferation of K562 may related with downregulation of Bcl-2.
出处
《现代生物医学进展》
CAS
2010年第13期2435-2438,共4页
Progress in Modern Biomedicine
基金
湖南省自然科学基金资助项目(05JJ30042)