强啡肽原基因3'非翻译区多态性位点与海洛因依赖的关联研究

Association between Polymorphism in 3' Untranslated Regions of Prodynorphin Gene and Heroin Dependence

目的:探讨强啡肽原(prodynorphin,PDYN)基因3'非翻译区的3个单核苷酸多态性(Single Nucleotide Polymorphism,SNP)位点与海洛因依赖的相关性。方法:严格按照诊断标准,选取无亲缘关系的海洛因依赖患者212例,健康对照200例提取基因组DNA,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测PDYN基因3'非翻译区rs1022563、rs2235749、rs9100803个多态性位点的基因型,采用HaploView及SPSS11.5软件分析各位点基因型、等位基因频率及组间差异。结果:PDYN基因rs2235749的基因型及等位基因频率分布在海洛因依赖组与正常对照组存在显著性差异(p<0.01),在海洛因依赖组中等位基因A的频率显著高于正常对照组(p<0.01)。连锁不平衡结果显示,这3个SNPs位点不连锁,D'=0.215。结论:PDYN基因3'非翻译区rs2235749与海洛因相关联,携带有等位基因A的个体可能对海洛因更容易产生依赖。

Objective：To investigate the relationship between the three functional polymorphisms in the 3＇untranslated regions of prodynorphin（PDYN） Gene and heroin dependence.Methods：Genomic DNA was isolated from the venous blood leukocytes of 212 unrelated patients with heroin dependence and 200 healthy unrelated individuals.Polymorphism of PDYN,rs1022563,rs2235749,rs910080 were analysed by PCR-RFLP（restriction fragment length polymorphism）.Genotype and allele frequencies were analysed by HaploView and SPSS11.5 software.Results：There was significant difference for both allele and genotype frequencies of PDYN rs2235749 between the study group and control group.Linkage disequilibrium showed that three SNPs,non-chain,D ＇= 0.215.Conclusions：There was relationship between the PDYN rs2235749 polymorphism and heroin dependence.The individuals with A allele are susceptible to heroin dependence.