外源性FHIT基因对人乳腺癌MDA-MB-436细胞恶性表型的影响

Effects of exogenous FHIT gene on malignant phenotype of human breast cancer cell line MDA-MB-436

目的:研究外源性脆性组氨酸三联体(FHIT)基因的表达对乳腺癌MDA-MB-436细胞增殖的影响并探讨其机制。方法:通过脂质体将外源性FHIT基因的真核表达质粒PEGFP-FHIT和空载体分别转染FHIT表达缺失的乳腺癌细胞MDA-MB-436。MTT法分析细胞增殖,流式细胞术观察细胞的凋亡,蛋白质印迹法检测外源性FHIT基因及凋亡相关基因Caspase-3、-8、-9的蛋白表达。结果:PEGFP-FHIT组细胞的生长抑制率和凋亡率明显高于空载体组和对照组,PEGFP-FHIT组细胞Caspase-3、-9活性片段表达上调。结论:外源性FHIT基因表达能抑制MDA-MB-436细胞增殖,其机制可能是通过激活Caspase途径从而诱导细胞凋亡。

OBJECTIVE： To investigate the effects of the FHIT gene on the malignant growth of breast cancer cell line MDA-MB-436 and its mechanism of cancer inhibition.METHODSD： Mammaliam expression vector PEGFP-FHIT was transfected into the breast cancer cell line MDA-MB-436 by liprofectamine.The effect of FHIT on the growth of MDA-MB-436 was examined by MTT and flow cytometry.The expressions of exogenous FHIT protein and Caspase-3,-8,-9 were detected by Western blot.RESULTS： The growth inhibitory rate and apoptosis rate of the cells in PEGFP-FHIT group were significantly higher than those in the PEGFP group and control group.The expressions of caspase-3,-9 active proteins were significant increased in the PEGFP-FHIT group.CONCLUSION：The expressions of exogenous FHIT gene can obviously suppress the proliferation and induce apoptosis by the activation of the caspase pathway in MDA-MB-436 cells.