摘要
生物材料的玻璃化低温保存技术已受到广泛注意。本文修正了Foutron的半经验结晶动力学模型,并用由此得到的解析表达式,构造各种浓度溶液在等温转变时的温度-时间-转变(TTT)曲线和变温转变时的连续-冷却-转变(CCT)曲线,预测实现玻璃化转变所需的最小冷却速率,以反映溶液玻璃化形成倾向的大小。结果表明,丙二醇的玻璃化形成倾向大于乙二醇和甘油。若在丙二醇溶液中加入丁二醇,可进一步提高其玻璃化形成能力。
In order to avoid the damage effects of ice formation during freezing, the vitrification technique for cryopreservation of the biological materials has arrested much public attention recently. In this paper, a semi-empirical model developed by Boutron has been modified. The exact analytical expressions obtained here can be used to construct those temperature-time-transformation(TTT)curves for isothermal crystalli- zation and continuous-cooling-transformation (CCT)curves for nonisothermal crystallization, from which the critical cooling rates required for vitrification of cryoprotective solutions can then be predicted directly. It is found that the glass-forming tendency of 1, 2-propanediol is higher than ethylere glycol and glycerol, the critical cooling rates can be reduced by adding 1,2-butanediol into 1,2-propanediol solution.
基金
国家自然科学基金
关键词
低温抗冻剂
结晶动力学
生物材料
Cryopreservarion
Cryoprotectant
Crystallization kinetics
Vitrification
