CyclinE干扰RNA对大鼠PC12细胞增值的影响

The Effects of SiRNA-Cyclin E on Cell proliferation in PC12 Cell Line

目的利用脂质体法转染CyclinE的干扰RNA真核表达载体,然后检测细胞的增值速率,探讨CyclinE在神经细胞中的作用,为帕金森病的研究提供有价值的资料。方法 1.利用脂质体法转染CyclinE的干扰RNA真核表达载体,用倒置荧光显微镜观察荧光蛋白表达量。2.利用MTT法检测转染后细胞的增值速率。结果 1.倒置荧光显微镜观察显示,成功的转染了CyclinE的干扰RNA真核表达载体。2.MTT法检测显示,转染组细胞的增值速率明显低于未转染组和阴性对照组。结论 RNA干扰CyclinE后使得PC12细胞的增值速率减慢。

Objective Eukaryotic expression vector of RNA interference specific for CyclinE was transfected to inhibit the expression of CyclinE in PC12 cell.It is critical to research the role that MPPs play in the nerve cells and provide valuable theoretical basis for the parkinsonism.Method 1.PC12 cell line was transfected with siRNA vectors above via oligofectamine,and green fluorescent protein contents was assayed by fluorescence microscopy.2.The auxodrome of transfection cell line was assayed.Result 1.eukaryotic expression vector of RNA interference specific for CyclinE was transfected into PC12 cells.2.Transfected PC 12cell line grows slow than others.Conclusion CyclinE siRNA can significantly inhibit proliferation of PC 12 cells.