PCR与培养鉴定法在口腔厌氧菌检测中的应用与对比

Application and Contrasting Analysis of PCR and Cultural Methods in Oral Anaerobic Bacteria detection

目的探讨聚合酶链式反应（PCR）检测口腔厌氧茼的优势。方法培养方法对口腔厌氧菌的培养与鉴定分析；PCR方法扩增口腔厌氧菌16SrDNA序列和七种厌氧菌特异性序列；同时采用厌氧菌标准菌株作对照。结果PCR和培养方法具有相同的厌氧菌检出率，均为100％（标准菌株为5／5，标本为18／18）；PCR和培养方法具有不同的鉴定准确率，标准菌株分别为100％（5／5）和40％（2／5）；标本PCR为66．7％（12／18）培养方法不易界定；两种方法鉴定结果一致率低，只有5．6％（1／18）。结论PCR在口腔厌氧菌检出和鉴定方面明显优于培养方法。

ObjectiveWe studied PCR＇s advantages in detecting the oral anaero bicbacteria. Methods The anaerobic bacteria in oral infected samples were detected and identified by the cultural identified method; Anaerobic bacteria 16S rDNA and 7 specific DNA sequences were detectedby PCR method. The standard＇ s anaerobic bacteria were detected by the same method simultaneously. Results The PCR and cultural methods had same anaerobic bacteria＇s detecting rate, they were 100% （the standard＇ s anaerobic bacteria were 5/5, the anaerobic bacteria in oral infected sampleswere 18/18 ） ; The two methods had deferent anaerobic bacteria＇ s identifying rate, they were 100 % （5/5） and 40 % （ 2/5 ） of the standard＇ s anaerobic bacteria.66.7% （12/18） of the oral infected sample by PCR and difficult determined by cultural method;The coincidence of the identifying rate by two methods were low, that was 5.6% （1/18）. Conclusion PCR method was a better method for detecting and identifying oral anaerobic bacteria.