APL患者诱导分化治疗前后粘附分子ICAM1和VCAM1变化与临床意义

The change and clinical siginficance of adhesive molecule ICAM1 and VCAM1 in acute promyelocytic leukemia cells induced by arsenic trioxide

目的探讨粘附分子sICAM-1和sVCAM-1与白血病之间的关系及其在白血病诊断、治疗及预后监测中的临床意义。方法采用ELISA法检测As2O3对原代APL细胞分泌sICAM-1和sVCAM-1的影响：通过RT—PCR检测As203治疗前后APL患者sICAM-1和sVCAM—1 mRNA表达变化；采用MTT法检测0．5umol／L的As2O3对APL细胞体外增殖的影响。结果As2O3治疗前APL患者血清中粘附分子水平显著高于对照组，治疗后5～12天血清sICAM-1水平升高，此后逐渐下降，30天时接近正常水平。As2q在体外也可升高原代APL细胞分泌粘附分子水平。As2O3治疗后sVCAM—1和sICAM-1的基因表达均增强，并且随着治疗时间的延长，表达量上调。As2O3在体外能显著抑制APL原代细胞的增殖。结论三氧化二砷可诱导APL细胞由早幼粒细胞向成熟细胞分化，诱导APL患者血清中sVCAM-1和sICAM—1分泌。

Objective Aims To detect the relationship between adhesion molecuars slCAM- 1 or sVCAM- 1 and APL. Methods ELISA and FCM methods were uesd to detect the secretion level of adhesive molecules. MTT assay was used to detectthe proliferation of APL primary cells. RT - PCR was used to detect the rnRNA expression of slCAM - 1 and sVCAM - 1. Results Seeration of adhesive molecules was up - regulated by As2O3 both in APL patients and in APL cells. The mRNA expression of them up regulated too. Furthermore, proliferation of the APL cells could be inhibited by As2O3 in vitro. Conclusions Therapy with As2O3 could increase the section level of sVCAM - 1, slCAM- 1, CDllb, and also up - regulate the mRNA expression of them.