三种中药逆转大肠杆菌耐药性的实验观察

The drug - resistant reversal effect of three Chinese medicines on E. coli

目的研究三黄汤、大黄、小檗碱对抗庆大霉素大肠杆菌的抑制作用及耐药性逆转作用。方法用二倍稀释法在96孔板中测定三黄汤、大黄、小檗碱对大肠杆菌的半数致死浓度；检测三种中药和庆大霉素联合用药效果；将大肠杆菌耐药株分别在三种中药中连续传代后检测对庆大霉素的敏感性变化，计算耐药性逆转倍数。结果庆大霉素对大肠杆菌质控菌株和耐药株的EC50分别为3．5mg／ml和50mg/ml，三黄汤、大黄、小檗碱对大肠杆菌质控菌株和耐药株的EC50差别不显著；中药和庆大霉素联用与单用相比对大肠杆菌的抑制作用提高不显著；在中药中连续传代后，大肠杆菌对庆大霉素的敏感性均有提高，在三黄汤中传代后提高尤为显著，耐药性逆转倍数为2．57。结论将大肠杆菌在三黄汤中连续传代可逆转其耐药性。

Objective to detect the reveral effect of drug- resistant E. coli to gentamicin by san huang tang, rheum officinale baill and berberine. Methods Double dilution method was used to test EC50 of san huang tang, rheum officinale baill and berberine to E. coli. Combinationg effects of gcntamicin and three Chinese medicines were tested. To work out the reverse factor, EC50 of gentamicin to the passaged E. coli in san huang tang, rheum officinale baill and berberine was tested. Results EC50 of gentamicin to E.coli ATCC25922 and GR were 3.5 mg/ml and 50 mg/ml respectively. EC50 to E. coli ATCC25922 and GR was samely generated by san huang tang, rheum officinale baill and berberine. There was no synergic effect between gentamicin and three Chinese medicines. Sensitivity. of the passaged E. eoli in san huang tang was improved significantly. Conclution The drug- resistant effect can be reversed by san huang tang.