自然杀伤细胞对多发性骨髓瘤细胞株KM-3影响的研究

Impact of NK cells on multiple myeloma cell line KM-3

目的 研究自然杀伤（NK）细胞对多发性骨髓瘤细胞株KM-3杀伤及诱导凋亡的作用.方法 WST-1法观察不同效靶比的NK细胞对KM-3细胞的杀伤作用;流式细胞术检测Annexin-V＋/PI-凋亡细胞、线粒体跨膜电位.结果 NK细胞作用于KM-3细胞后,能显著杀伤KM-3细胞,呈剂量和时间依赖性（P〈0.05）;NK细胞作用于KM-3细胞48 h后,Annexin-V＋/PI-细胞比例明显增加,呈剂量依赖性（P〈0.05）;NK细胞作用于KM-3细胞效靶比为10：1时,Annexin-V＋/PI-细胞比例明显增加,呈时间依赖性（P〈0.05）;NK细胞作用于KM-3细胞后,KM-3细胞线粒体跨膜电位明显降低,呈剂量和时间依赖性（P〈0.05）.结论 NK细胞能明显杀伤KM-3细胞并诱导其凋亡,均呈剂量和时间依赖性.

Objective To study the apoptosis of multiple myeloma cell line KM-3 induced by NK cells. Methods WST-1 assay was used to detect the killing effect of KM-3 cells treated with NK cells at different effector（E）：target（T） ratio. Flow cytometry was applied to analyze Annexin-V＋/PI- apoptotic cells and the mitochondrial transmembrane potential. Results NK cells could significantly kill KM-3 cells in a dosand time-dependent manner （P 〈0.05）. After KM-3 cells- were treated with NK cells for 48 hours, the Annexin-V＋/PI- cells were increased obviously in dose-dependence （P 〈0.05）. The Annexin-V＋PI- cells were increased in time-dependence when treated with NK cells（E：T ratio at 10：1） （P〈0.05）. The mitochondrial transmembrane potential of KM-3 cells treated with NK cells were significantly decreased in dose-and time-dependence （P 〈 0.05）. Conclusion NK cell can kill KM-3 cells and induce apoptosis in a dose-and time-dependence manner.