基质金属蛋白酶MMP-1和MMP-2与喉癌侵袭和淋巴结转移的关系

Expression of matrix metalloproteinases(MMP-1 and MMP-2) and local invasion and lymphoid metastasis of laryngeal carcinoma

目的研究基质金属蛋白酶MMP-1和MMP-2在喉癌组织中的表达及其与喉癌局部侵袭和淋巴结转移的关系。方法采用免疫组织化学SABC法检测30例喉癌和10例声带息肉标本的MMP-1和MMP-2表达,结合喉癌患者的分期、淋巴结转移和临床资料等进行统计分析;同时采用免疫组织化学SABC法检测Hep-2细胞株和裸鼠移植瘤中的MMP-1和MMP-2表达。结果喉癌组织中MMP-1和MMP-2的阳性表达率分别为76.7%(23/30)和80.0%(24/30),均明显高于声带息肉组,两者比较均差异有显著性(P<0.05);喉癌中T3～4分期组MMP-1、MMP-2的阳性表达率均明显高于T1～2组(P<0.05);有淋巴结转移和无淋巴结转移者之间的MMP-1和MMP-2阳性表达率均差异有显著性(P<0.05)。MMP-1和MMP-2的表达呈正相关(r=0.694,P<0.01)。性别、年龄等对MMP-1和MMP-2的表达没有影响(P>0.05)。Hep-2细胞涂片中MMP-1和MMP-2阳性细胞数均大于75%。裸鼠移植瘤切片见癌巢高表达MMP-1和MMP-2。结论 MMP-1、MMP-2在喉癌细胞中高表达,其表达程度与喉癌的局部侵袭和淋巴转移具有密切关系,因此MMP-1和MMP-2的检测可以成为临床判断喉癌的恶性程度、侵袭能力及淋巴结转的重要参考指标,具有重要临床意义。

[Objective] To investigate the relationship between the expression of matrix metalloproteinases（MMP- 1 and MMP-2） with local invasion and lymphoid metastasis of laryngeal carcinoma. [Methods] Expression of MMP-1 and MMP-2 in 40 cases of laryngeal carcinoma and 10 cases of vocal cord polyp were detected by immunohistoehemistry, and their relationship with stage, lymphoid metastasis and clinical data were analyzed. Expression of MMP-1 and MMP-2 both in Hep-2 cells and in xenografted nude mice were also detected by immunohistochemistry. [Results] The positive rate of MMP-1 and MMP-2 in laryngeal carcinoma was 76.7% （23/30） and 80% （24/ 30） respeetively, which were significantly higher than those in vocal cord polyp（P 〈0.05）. The positive rate of MMP- 1 and MMP-2 in laryngeal carcinoma with Tr4 were both significantly higher than those with T1-2（P 〈0.05）; and there was a statistical signifieant difference between the positive rate of MMP-1 with and without lymphoid metastasis （P 〈 0.05）, the same as MMP-2. There is a oositive eorrelation between MMP-1 and MMP-2. the correlation coefficient is 0.694（P 〈0.05）. Aging and sender had no affect on expression of MMP-1 or MMP-2（P 〉0.05）. The MMP-1 posi- tive cells and MMP-2 positive cells were more than 75% in Hep-2 cells smear. A high expression of MMP-1 and MMP-2 were seen in cancer nest of xenografted nude mice. [Conclusions] There is a high expression of MMP-1 and MMP-2 in laryngeal carcinoma ceils, and the expression has a close correlation with local invasion and lymphoid metastasis of laryngeal carcinoma. Detection of MMP-1 and MMP-2 can be used as a index to predict malignant degree, invasive ability and lymphoid metastasis, which has important clinical significance.