桦褐孔菌多糖对二乙基亚硝胺致肝脏损伤的保护作用

The Protective Effect of Inonotus Obliquus Polysaccharide (IOP) on the Hepatic Injury Induced by Diethylnitrosamine

[目的]研究桦褐孔菌多糖(inonotus obliquus polysaccharide)对二乙基亚硝胺(DEN)致肝脏损伤的保护作用。[方法]将50只小鼠随机分为5组:阴性对照组(蒸馏水),DEN组(20 mg/kg),桦褐孔菌多糖高(600 mg/kg)、中(300 mg/kg)、低(150 mg/kg)剂量组。阴性对照组及桦褐孔菌3个剂量组每日灌胃,同时DEN组和桦褐孔菌3个剂量组隔日腹腔注射DEN,持续5周。采用HE染色法观察动物肝细胞核分裂相及枯否细胞数;采用酶动力学法测定肝组织匀浆上清液中谷胱甘肽-S-转移酶(GSTs)和微量丙二醛(MDA)含量;采用酶联免疫吸附法(ELISA)检测肝组织匀浆上清液中肿瘤坏死因子α(TNF-α)含量。[结果]DEN组肝组织中核分裂相和枯否细胞数明显多于阴性对照组(P<0.01),桦褐孔菌多糖高剂量组肝组织中核分裂相和枯否细胞数显著低于DEN组(P<0.05)。DEN组肝组织中GSTs活性明显低于阴性对照组(P<0.05),MDA和TNF-α含量明显高于阴性对照组(P<0.01或P<0.05)。桦褐孔菌多糖高剂量组和中剂量组肝组织中GSTs活性明显高于DEN组(P<0.01),3个剂量组肝组织中MDA含量均显著低于DEN组(P<0.01或P<0.05),只有高剂量组肝组织中TNF-α含量明显低于DEN组(P<0.05)。[结论]桦褐孔菌多糖对DEN致肝脏损伤有一定的保护作用。

[ Objective ] To study the preventive effect of inonotus obliquus polysaccharide（ IOP ）on the hepatic injury induced by diethylnitrosamine（ DEN ）. [ Methods ] Fifty mice were randomly divided into five groups： negative control group（ Distilled water ）, DEN group（ 20mg/kg ）, high dose of IOP group（ 600mg/kg ）, middle dose of IOP group（ 300 mg/kg ）, and low dose of IOP group（ 150mg/ kg ）. Mice of negative control group were given with distilled water and the three IOP groups were given with IOP, all by gavage daily for 5 weeks. Mice of DEN group and three IOP groups were given with DEN intraperitoneally every other day. After 5 weeks, the mice were killed, the number of liver cell mitosis and Kupffer cell were counted under microscope with HE staining. The activity of glutathione S-transferases （ GSTs ） and content of malondialdehyde （ MDA ） were determined by enzyme dynamic method and the content of tumor necrosis factor α （ TNF-α ）in liver tissue was determined by ELISA. [ Results ] The numbers of mitosis and Kuffer cell in DEN group were more than that of the negative control group with significant statistical difference（ P 〈 0.01 ）, and the high dose of IOP group showed less mitosis and Kuffer cell than that of the DEN group with significant statistical difference（ P 〈 0.05 ）. The activity of GSTs in DEN group was lower than that of the negative control group with significant statistical difference（ P 〈 0.05 ）, the contents of MDA and TNF-α in DEN group were higher than that of the negative control group with significant statistical difference（ P 〈 0.01 or P 〈 0.05 ）. The activities of GSTs in the high dose and middle dose of IOP group were higher than that of the DEN group with significant statistical difference （ P 〈 0.01 ）. The contents of MDA in three different dose of IOP groups were lower than that of DEN group with significant statistical difference（ P 〈 0.01 or P 〈 0.05 ）. Only in high dose of lOP group the content of TNF-α was significantly lower than that of DEN group with significant statistical difference （ P 〈 0.05 ）. [ Conclusion ] IOP has some protective effect on the hepatic injury induced by DEN.