TGF-β1 shRNA对涎腺人黏液表皮样癌裸鼠颌下腺移植瘤的抑瘤作用及Ki-67、VEGF蛋白表达的影响

Inhibitory Effect of TGF-β1 shRNA on Human Salivary Gland Mucoepidermoid Carcinoma in Nude Mice Submandibular Gland and Effect of Ki-67 and VEGF Expression

目的研究TGF-β1 shRNA对人涎腺黏液表皮样癌(Ms)细胞裸鼠颌下腺原位移植瘤成瘤能力以及对Ki-67和VEGF蛋白表达的影响。方法将Ms细胞、稳定转染空载体的Ms细胞、稳定转染TGF-β1 shRNA的Ms细胞分别接种至裸鼠颌下腺,观察各组细胞在裸鼠体内的成瘤率,测量肿瘤体积、瘤质量并绘制肿瘤生长曲线;免疫荧光法和免疫组织化学法检测肿瘤组织中Ki-67和VEGF蛋白的表达。结果与未转染组、转染空载体组相比,转染TGF-β1 shRNA组裸鼠颌下腺原位移植瘤生长缓慢,瘤体体积和瘤质量显著降低(P<0.01),瘤细胞中Ki-67和VEGF蛋白表达明显降低。结论 TGF-β1 shRNA能显著抑制Ms细胞在体内的生长能力,其机制可能与下调细胞内Ki-67和VEGF水平有关。

Objective To study inhibitory effect of TGF-β1 shRNA on human salivary gland mucoepidermoid carcinoma in nude mice submandibular gland and the effect of Ki-67 and VEGF expression.Methods Nude mice submandibular gland were transplanted with Ms cells,Ms cells stably transfected with empty vectors,Ms cells stably transfected with TGF-β1 shRNA,respectively.The tumor volume and weight were measured and observed by growth curve.Immunohistochemistry and immunoflurescence were used to detect the level of Ki-67 and VEGF.Results Compared with Ms cells and Ms transfected with empty vectors,Ms cells stably transfected with TGF-β1 shRNA showed slow growth in tumor volume and weight （P〈0.01）,as well as down-regulated the expressions of Ki-67 and VEGF.Conclusion TGF-β1 shRNA could inhibit the tumor growth of Ms cells,the mechanism of which might be related with down-regulation of Ki-67 and VEGF.