RNAi下调hTERT基因对MCF-7乳腺癌细胞生长的影响

Effect of Targeting hTERT Gene Inhibited in MCF-7 Breast Cancer Cell Growth by RNA Interference

目的利用si RNA在乳腺癌细胞内诱导RNAi,抑制人端粒酶催化亚基(human telomerasereverse transcriptase,hTERT)基因表达,在体外细胞水平探讨RNAi对MCF-7乳腺癌细胞生长的影响。方法构建靶向hTERT基因的siRNA(hTERT-si RNA),转导入MCF-7乳腺癌细胞,在瘤细胞内诱导RNAi,采用细胞增殖抑制实验、RT-PCR法、Western blot等技术检测si RNA处理前后瘤细胞增殖及hTERT基因表达变化。结果 hTERT-si RNA对乳腺癌细胞生长抑制率达61.94%;hTERT的mRNA表达降低了61.7%,蛋白表达平均降低了52.8%。结论 si RNA在体外明显抑制了乳腺癌细胞中hTERT基因的表达和瘤细胞增殖。

Objective To investigate the effect of breast cancer cell growth,we utilized RNAi tragedy to inhibit hTERT gene in MCF-7 cells of breast cancer. Methods Small intererence RNA （siRNA） homologous to hTERT gene （hTERT-siRNA） was recombined with pGenesil-1 vector,then transfected into MCF-7 cells of breast cancer to induce RNAi,and then hTERT gene expression alterations and tumor cell proliferation in both hTERT-siRNA treatment groups and control groups were detected by MTT assay,RT-PCR and Western blot. Results The expression of hTERT had been obviously inhibited by siRNA.The inhibition rate of cell proliferation was 61.94%.The mRNA level of hTERT was obviously decreased by 61.7%,and its protein expression reduced by 52.8%. Conclusion siRNA-hTERT effectively inhibited hTERT gene expression and breast cancer cell proliferation in vitro.