Prox1在乳腺癌组织中的表达及其与淋巴道转移的关系

Expression of Prox1 in Human Breast Cancer and Its Relationship with Tumor Lymphatic Metastasis

目的探讨Prox1在乳腺癌组织中的表达及其与各临床病理因素的关系。方法采用免疫组织化学SP法检测11例良性乳腺增生组织和80例浸润性乳腺导管癌组织(其中30例合并转移淋巴结)中Prox1的表达。结果 Prox1在良性乳腺增生组织中均为阴性表达,在乳腺癌组织中癌细胞和淋巴管内皮细胞的阳性表达率分别为62.5%(50/80)和65.0%(52/80),其中有淋巴结转移组阳性率分别为76.7%(23/30)和83.3%(25/30),明显高于无淋巴结转移组阳性率54.0%(27/50)和54.0%(27/50),两组间的差异有统计学意义(P<0.05);且在有转移组,淋巴管内皮细胞Prox1的表达与淋巴结转移数目有关(P<0.05),但其表达与ER、PR、HER-2以及年龄无关。在乳腺癌组织中癌细胞与淋巴管内皮细胞Prox1的表达呈正相关(r=0.452,P<0.001),且二者的表达均与TNM分期呈正相关(r=0.589,P<0.001)。结论 Prox1在乳腺癌组织中的高表达可能与乳腺癌淋巴道转移密切相关。

Objective To investigate the expression of Prox1 in human breast carcinoma and its relationship with clinical pathologic characters.Methods The expression of Prox1 was detected by SP immunohistochemistry in 11 specimens of hyperplasia of mammary glands,80 specimens of breast cancer （30 with lymphatic metastasis）.Results The expression of Prox1 in 11 specimens of hyperplasia of mammary glands was completely negative,but were 62.5%（50/80）of the carcinoma nests cells and 65.0%（52/80）of lymphatic endothelial cells in breast cancer specimens.The percentage of cells positive for Prox1 in breast cancer patients with metastasis （76.7%,23/30） was higher than those without （54.0%,27/50）,as well as in lymphatic endothelial cells with metastasis （83.3%,25/30） than those without （54.0%,27/50）.The differences of two groups have statistical significance （P 0.05 ）.The expression of Prox1 in the lymphatic endothelial cells of lymphatic metastasis specimens was correlated with the numbers of invaded lymph nodes（P〈0.05）.However,no significant correlation was found between Prox1 expression level and the following clinical parameters：age,ER status,PR status,HER2 status（P〉0.05）.Positive correlation（r=0.452,P〈0.001）of the Prox1 expression was found between carcinoma cells and lymphatic endothelial cells,and Prox1 expression in carcinoma cells and lymphatic endothelial cells were both positively correlated with TNM stage（r=0.589,P〈0.001）.Conclusion The high expression of Prox1 may be correlated with the process of breast cancer progression and metastasis.