肝细胞癌中OY-TES-1基因mRNA的表达

OY-TES-1 Gene mRNA Expression in Human Hepatocellular Carcinoma Tissues

目的检测OY-TES-1基因mRNA在肝细胞癌(HCC)中的表达水平。方法建立实时定量PCR方法 ,检测56例HCC及37例配对癌旁组织中目的基因OY-TES-1及看家基因HPRT的表达,OY-TES-1 mRNA的表达量以OY-TES-1/HPRT表示,应用SPSS13.0统计分析软件进行数据分析。结果 (1)经实时定量PCR检测,HCC中OY-TES-1 mRNA阳性率为73.21%(41/56),癌旁组织阳性率为64.86%(24/37),有17对HCC及配对癌旁组织均表达阳性,OY-TES-1 mRNA在HCC及癌旁组织中的阳性表达率差异无统计学意义(P>0.05);(2)HCC中OY-TES-1 mRNA水平明显高于对应的癌旁组织(P<0.05),但与HCC患者的年龄、性别等临床资料无关。结论 OY-TES-1 mRNA在HCC中有较高的表达频率,其表达水平高于对应的癌旁组织,它有望作为用于HCC辅助诊断及免疫治疗的肿瘤抗原。OY-TES-1蛋白在HCC及癌旁组织中的表达及相关意义尚值得做进一步的研究。

Objective To investigate the expression level of OY-TES-1 mRNA in human hepatocellular carcinoma （HCC）tissues.Methods To establish quantitative real-time polymerase chain reaction for quantitative expression of OY-TES-1 mRNA.Fifty-six specimens of human HCC tissues and thirty-seven specimens of adjacent HCC tissues were analyzed for quantitative expression of target gene OY-TES-1 and housekeeping gene HPRT. OY-TES-1/HPRT was used to evaluate the expression level of OY-TES-1 mRNA.Data were dealt with statistical analysis software SPSS 13.0.Results （1） The expressive frequency of OY-TES-1 in HCC was 73.21%（41/56）while 64.86%（24/37）in adjacent HCC tissues by quantitative real-time PCR.Meanwhile,17 pairs show positive,which had no significant difference（P〉0.05）.（2） The expression level of OY-TES-1 mRNA in human HCC tissues was higher than that in adjacent HCC tissues（P〈0.05）.No relationship was found between the expression of OY-TES-1 genes and clinic pathological parameters （P〉0.05）.Conclusion With higher expressive frequency and level in human HCC,OY-TES-1 is a potential target for antigen-specific immunotherapy and auxiliary diagnosis of HCC.Our interest will now focus on the expression of OY-TES-1 protein in HCC and its significance.