大承气汤治疗对实验性急性胰腺炎大鼠炎症反应及氧化应激水平的影响

The effect of Dachengqi Decoction on inflammatory response and oxidative stress in rats with acute pancreatitis

目的:探讨大承气汤治疗对实验性急性胰腺炎大鼠炎症反应及氧化应激水平的影响。方法:将成年雄SD大鼠54只随机分为3组:假手术组、模型组和治疗组。经十二指肠行胆胰管逆行加压注射50 g/L牛磺胆酸钠建立大鼠急性胰腺炎模型。造模后治疗组立即给予大承气汤喷雾干燥颗粒20 g/kg灌胃治疗1次,模型组和假手术组给予等量的生理盐水。分别于造模后3、6、12 h处死每组各6只大鼠并立即心脏取血,ELISA方法测定血清中TNF-α、IL-β和IL-6含量;同时切取各时间点大鼠胰腺组织,检测丙二醛(malondialdehyde,MDA)、组织髓过氧化物酶(myeloperoxidase,MPO)及超氧化物歧化酶(superoxide dismutase,SOD)的含量。结果:与模型组相比,治疗组各时间点的血清TNF-α、IL-β、IL-6含量均显著降低(P<0.01或P<0.05);同时胰腺组织中MDA、MOP含量亦明显减少(P<0.05),而SOD含量则明显升高(P<0.05)。结论:大承气汤对于急性胰腺炎具有重要的治疗和保护作用,其机制可能与清除氧自由基,减轻脂质过氧化反应,以及减轻炎症反应相关。

Objective： To evaluate the effects of Dachengqi Decoction on inflammatory response and oxidative stress in AP rats（acute pancreatitis）.Methods： Fifty-four SD rats were randomized into three groups： sham-operation group,AP group and Daehengqi Decoction treated group.The models of AP were made by retrograde infusion of 50 g/L sodium taurocholate into the pancreatic duct of SD rats.The serum TNF-α,IL-βand IL-6 were measured and myeloperoxidase（MPO）,malondialdehyde（MDA） and superoxide dismutase（SOD） in pancreatic tissue were also detected 3,6 and 12 hours after operation.Results： Serum TNF-α,IL-βand IL-6 in serum and the tissue content MPO and MDA were significantly increased meanwhile SOD significantly decreased in AP group compared with those in sham-operation group（P0.05）.Compared with AP group,except SOD,the indexes were significantly decreased in Dachengqi Decoction treated groups（P0.05）.Conclusion： Dachengqi Decoction may have therapeutic and protective effects on AP by cleaning oxygen free radicals and decreasing inflammatory reactions.