钩藤水提物Batch-2对6-羟多巴胺诱导的SH-SY5Y细胞损伤的神经保护作用(英文)

The Neuroprotective Effect of Batch-2, an Aqueous Extract From Cat′s Claw (Uncaria tomentosa) on 6-OHDA-Induced SH-SY5Y Cell Damage

以前的药理学研究表明,钩藤水溶性提取物C-MED-100TM不仅具有抗氧化活性,而且还具有很好的DNA修复和免疫功能.Batch-2是一种新的水溶性钩藤提取物,而它的自由基清除能力及神经保护作用还未见报道.首先检测了batch-2对六羟多巴胺诱导的SH-SY5Y细胞损伤的神经保护作用机制,然后利用红外光谱、HPLC和分光光度技术对batch-2的组成成分进行了分析.结果表明,batch-2具有清除各种自由基的能力,尤其是对羟自由基的清除(25mg/L的batch-2对羟自由基的清除率为60%),batch-2可剂量依赖性地抑制6-羟多巴胺诱导的细胞凋亡、脂质过氧化水平、线粒体膜电位的降低和细胞内活性氧和一氧化氮的增加.同时,batch-2抑制了由6-羟多巴胺诱导的SH-SY5Y细胞内iNOS和NF-κB蛋白的上调.结果表明,batch-2对六羟多巴胺诱导的SH-SY5Y细胞损伤的神经保护作用是通过清除活性氧和一氧化氮、抑制iNOS和NF-κB表达实现的.成分分析表明,batch-2中的多酚和奎宁酸含量分别为6.43%和0.0958%.上述结果显示,batch-2的抗氧化机制部分类似于EGCG.对于帕金森病的预防,batch-2是一个潜在具有很好的神经保护作用的天然抗氧化剂.

Previous pharmacological studies found that a water-soluble extract （C-MED-100TM） of Uncaria tomentosa has antioxidant activity and beneficial effects on DNA repair and immune function. Batch-2 is a novel aqueous extract from cat’s claw, however, the free radical scavenging ability and neuroprotective effect of batch-2 have not been reported. Firstly, the neuroprotective effect of batch-2 on the 6-OHDA-induced SH-SY5Y cell damage was detected. Secondly, the component analysis of batch-2 was tested by infrared spectroscopy, HPLC and spectrophotometry.The results showed that batch-2 has scavenging ability to several kinds of free radicals, especial the hydroxyl radical （25 mg/L of batch-2 can scavenge 60% of the hydroxyl radical）. The dose dependently attenuated 6-OHDA-induced cell death, lipid peroxidation, mitochondrial potential loss and increase of intracellular reactive oxygen species （ROS） and nitric oxide （NO） in SH-SY5Y cells were also observed. Meanwhile, batch-2 can suppress the 6-OHDA induced increase of expression of iNOS and activity of NF-κB. The results show that the neuroprotective effect of batch-2 on 6-OHDA-induced damage of SH-SY5Y cells by scavenging ROS and NO, inhibiting activation of iNOS and NF-κB. Component analysis shown that the content of polyphenol and quininic acid from Batch-2 was 6.43% and 0.0958% respectively. These results provided evidence to support that the mechanism of batch-2 for antioxidative activity and neuroprotective effect may be similar with （-）-epigallocatechin-3-gallate（EGCG） partly. It is obvious that batch-2 is a potential neuroprotective antioxidant for the precaution candidate of Parkinson’s disease.