放疗联合热疗对肝癌细胞的协同杀伤作用

Synergistic killing effect of radiotherapy and thermotherapy on hepatoma cells in vitro

目的:研究放疗联合热疗对肝癌细胞有无协同杀伤作用,为临床应用提供实验依据.方法:以人肝癌细胞株HepG2为研究对象,实验分成4组:(1)对照组;(2)单纯热疗组;(3)单纯放疗组;(4)放疗与热疗联合组.各组分别进行实验.采用流式细胞术检测细胞凋亡情况,电镜观察肝癌细胞的超微结构,免疫组织化学检测P53蛋白表达变化.结果:与对照组相比,3个治疗组的肿瘤细胞的凋亡率均增加(19.98%±1.76%,20.83%±1.84%,32.16%±2.03%vs16.47%±1.24%,P<0.05或0.01),细胞超微结构损伤,P53蛋白的表达增加(34.98%±1.16%,35.21%±1.23%,45.50%±1.55%vs32.00%±1.05%,P<0.05或0.01);与单纯的放疗或热疗组相比,放疗与热疗联合组的肝癌细胞凋亡率明显增高(P<0.01),超微结构损伤更显著,P53蛋白的表达显著增加(P<0.01).结论:放疗联合热疗对肝癌细胞的杀伤有协同作用,其机制可能通过上调P53蛋白表达,诱导细胞凋亡实现的.

AIM：To investigate whether radiotherapy （RT） and thermotherapy （TT） have a synergistic killing effect on hepatoma cells （HepG2） in vitro.METHODS：Human HepG2 cells were divided into four groups：control group, TT group, RT group, and RT plus TT group.Cell apoptosis was determined by flow cytometry （FCM） assay.The ultrastructural alterations of HepG2 cells were observed under a transmission electron microscope.The expression of P53 protein was detected by immunohistochemistry.RESULS：Compared with the control group, the apoptosis rate significantly increased （19.98% ±1.76%, 20.83±1.84% and 32.16%±2.03% vs 16.47%±1.24%, all P0.05 or 0.01）, cell ultra-structural alterations were more signif icant, and the expression of P53 protein was significantly up-regulated （34.98%±1.16%, 35.21%±1.23%, 45.50%±1.55% vs 32.00%±1.05%, all P0.05 or 0.01） in the TT group, RT group, and RT plus TT group, which were most prominent in the RT plus TT group.CONCLUSION：RT and TT exert a synergistic killing effect on hepatoma cells possibly by upregulating P53 expression and inducing apoptosis.