资源植物罗布麻的愈伤组织诱导和植株再生研究

Callus Induction and Plantlet Regeneration Systems from a Resources Plant Apocynum venetum L.

以罗布麻(Apocynum venetum L.)无菌苗的叶、茎和根作为外植体,在不同激素与浓度组合的MS培养基上进行愈伤组织诱导和植株再生研究。结果表明,在多个激素浓度配比的培养基中,罗布麻叶和茎的愈伤组织诱导率均可达到100%;但进一步将愈伤组织转接到含有不同激素组合的培养基进行不定芽分化时,叶和茎来源的愈伤组织的不定芽分化率有很大不同,茎来源的愈伤组织虽可在多个培养基中有不定芽分化,但最高诱导率仅达20%;而叶片来源的愈伤组织虽仅有4个激素组合培养基中有不定芽的分化,但最高分化率可达到35%。因此,构建罗布麻再生体系的最佳外植体应选择叶片。愈伤组织诱导与不定芽分化的最佳培养基均为:MS+NAA0.1 mg/L+6-BA 1.0 mg/L;不定芽在1/2MS+NAA 0.2 mg/L生根培养基的生根效果最佳,不仅根群质量好,且生根率也高达100%;此再生苗的移栽成活率也最高,在适宜条件下可达75%。

To establish the regeneration systems for Apocynum venetum L.,leaves,stems,and roots from its aseptic seedlings were cultured in MS mediums with different concentration combinations of NAA and 6-BA.Results showed that both the callus inducement rates from leaves and stems could reach 100% on mediums with several combinations of NAA and 6-BA.When callus were transferred to induce adventitious buds,however,their differentiation rates were quite different.Adventitious buds could be found on callus from stems in several mediums,with the highest differentiation rate of 20%,but could only be found on the callus from leaves in four mediums,with the highest differentiation rate of 35%.The best explants for plan-tlet regeneration were leaves and the optimum medium for callus inducement and adventitious bud differentiation was MS＋NAA 0.1 mg/L＋ 6-BA 1.0 mg/L.The optimum root medium was 1/2 MS ＋ NAA 0.2 mg/L,within which the rooting rate could reach 100% and the quality of the root mass was the healthiest.Under suitable hardening and transplanting conditions,the highest survival rate for the regenerated plantlets reached 75%.