柚雌蕊不同部位抽提液对自交不亲和阻抑效果的研究

Effects of Water-soluble Extracts from Different Pistil Parts on Self-incompatibility Suppression in Pomlo(Citrus grandis)

以‘土柚’(Citrus grandis ‘Tuyou’)作对照,采用荧光显微技术对‘琯溪蜜柚’(Citrus grandis‘Guanxi-miyou’)、‘度尾蜜柚’(Citrus grandis ‘Duweimiyou’)两个品种花粉在本品种和异品种雌蕊不同部位抽提液中花粉萌发和花粉管伸长状态进行了观察。结果表明:‘琯溪蜜柚’和‘度尾蜜柚’子房抽提液对自身花粉管伸长阻抑最明显,并产生严重的弯曲;柱头抽提液对自身花粉管伸长稍有影响,出现中度弯曲;花柱抽提液中自身花粉管伸长正常,仅有轻微弯曲。然而,对于异品种的花粉以及自交亲和的‘土柚’,其子房、花柱和柱头抽提液对花粉管伸长没有这种阻抑效应。

Using Citrus grandis ＇Tuyou＇ as the control,pollen germination and pollen tube growth status of Citrus grandis ＇Guanximiyou＇ and ＇Duweimiyou＇ in water-soluble extracts from different pistil parts from the same and different varieties were observed by fluorescent microscopy.The results showed that pollen tube growth was retarded and bent seriously by the water-soluble extracts of ＇Guanximiyou＇ and ＇Duweimiyou＇ ovaries,and were also affected by stigma extracts with moderate bent.The pollen tube grew normally in their-own styles extracts,showing only a slight bend.However,there were no such suppression effects on pollen tube growth in the water-soluble extracts of stigmas,styles,and ovaries for the pollens of different varieties and self-compatible variety ＇Tuyou＇.