春季北黄海表层海水中溶解游离氨基酸的分布与组成研究

Study on Dissolved Free Amino Acid(DFAA)in the Surface Water of the North Yellow Sea

于2007年4月用高效液相色谱法分析北黄海42个站位表层海水中溶解游离氨基酸(DFAA)浓度的分布特征及组成。本次调查还分析了Chl a的浓度分布以及DFAA与Chl a之间的关系。结果表明:北黄海表层海水中DFAA浓度的变化范围为0.19～1.15μmol·L^(-1),平均浓度为(0.52±0.24)μmol·L^(-1);Chl a的浓度变化范围为0.24～4.76μg·L^(-1),平均值为(1.02±0.81)μg·L^(-1)。总体看来含量最多的个体氨基酸分别为丝氨酸、天门冬氨酸、甘氨酸、丙氨酸、缬氨酸、苯丙氨酸、苏氨酸,它们占DFAA含量的70.7%;而在高Chl a浓度与低Chl a浓度站位间DFAA的组成存在较大差异。本次调查发现DFAA与Chl a浓度间存在一定的相关性(R=0.344,n=42,P<0.05)。

A high performance liquid chromatography （HPLC） method was applied to measure the concentrations and composition of dissolved free amino acids （DFAA） in the surface water samples collected from 42 stations located in the North Yellow Sea, China in April 2007. The concentrations of chlorophyll a and the correlation between the chlorophyll a and DFAA were also analyzed. The results showed that： the DFAA in the North Yellow Sea surface in the range of 0. 19 to 1.15 μmol ·L^-1, the average concent ration of （0. 52±0. 24） μmol·L^-1, and chlorophyll a in the range of 0. 24 to 4. 76 μg·L^-1, the average concentration of （1.02±0. 81） μg·L^-1. Major constituents of DFAA present in the study area were serine, aspartic acid, glycine, alanine, valine, phenylalanine, and threonine, occupying about 70. 7% of total DFAA. The composition of DFAA varied with chlorophyll a concentrations at different stations. In this study, a correlation was found between DFAA and chlorophyll a （R=0. 344, n=42, P〈0. 05）.