摘要
目的 探讨IL-17肝内表达与慢性HBV感染所致肝纤维化的相关性.方法 免疫组织化学法测定30例慢性HBV携带者、55例慢性乙型肝炎患者、20例乙型肝炎肝硬化患者肝组织内不同炎症程度分级和肝纤维化分期中IL-17的表达,ELISA法测定血清IL-17及肝纤维化指标HA、LN、PCⅢ、ⅣC的水平.组间差异性检验采用Kruskal-Wallis检验和Mann-Whitney检验,相关性分析采用Spearman分析.结果 肝组织IL-17表达水平在肝硬化组高于慢性乙型肝炎组（x2=25.3982,P=0.004）,在慢性乙型肝炎组高于慢性HBV携带组（x2=11.5056,P=0.001）;不同炎症程度及纤维化分级与IL-17表达强度呈正相关（r=0.718、0.693,均P＜0.01）;肝组织IL-17主要集中于汇管区,其表达强度与血清HA、LN、PCⅢ、ⅣC呈正相关（r=0.793、0.834、0.722、0.883,均P＜0.01）.结论 IL-17的肝内表达与肝内炎症程度分级及肝纤维化程度密切相关.
Objective To explore the relationship between intrahepatic expression of interleukin-17 （IL-17） and liver fibrosis in patients with chronic hepatitis B virus infection. Methods IL-17 expressions in livers with different inflammation activity grades and hepatic fibrosis stages from patients with chronic hepatitis B virus carriers （n= 30）, chronic hepatitis B （CHB, n = 55）, liver cirrhosis （LC, n=20） were measured by immunohistochemistry. Serum IL-17 and liver fibrosis indices of haluronic acid （HA）, laminin （LN）, type Ⅲ procollagen （PC Ⅲ ） and type Ⅳ collagen （ Ⅳ C） were determined by enzyme linked immunosorbent assay （ELISA）. The differences between groups were compared by Kruskal-Wallis test, and the Mann-Whitney test, and the correlation analysis was done by Spearman test. Results Intrahepatic IL-17 expression in LC group was significantly higher than CHB group （x2 =25. 3982, P=0. 004）, and that in CHB group was higher than chronic hepatitis B virus carriers group （x2 = 11. 5056, P= 0. 001）. The inflammation activity grade and hepatic fibrosis stage were both positively correlated with IL-17 expression （r= 0.718, 0. 693, respectively; both P〈0.01）. IL-17 mainly located in portal area and the expression was positively correlated with serum levels of HA, LN, PCⅢ and ⅣC （r=0. 793, 0. 834, 0. 722, 0. 883, respectively; all P〈0.01）.Conclusion Intrahepatic IL-17 expression is closely correlated with liver inflammation activity grade and hepatic fibrosis stage.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2010年第6期359-363,共5页
Chinese Journal of Infectious Diseases
