门脉高压肝脏小叶间动脉与小叶间静脉血管内皮生长因子受体的分布

Distribution of vascular endothelial growth factor receptor in hepatic arteries and veins of rats with portal hypertension

目的:明确肝硬化门脉高压前后VEGFR-1、2在肝脏血管的分布变化.方法:SD♂大鼠30只,体质量180-220g.动物随机分为肝硬化门脉高压模型组(n=20)和随正常对照组(n=10).正常对照组正常饮水饮食,实验组采用硫代乙酰胺皮下注射法制作肝硬化门脉高压模型.12wk后经组织病理学检查证实肝脏有假小叶形成和门静脉压力大于1.57kPa的大鼠共12只纳入本研究.采用免疫组织化学染色法,分别观察VEGFR-1和VEGFR-2的变化特点.结果:VEGFR-2在肝硬化门静脉高压组大鼠的小叶间动脉和静脉中的表达均显著高于正常对照组(t=24.306,54.776,均P<0.05).VEGFR-1在肝硬化门静脉高压组大鼠的小叶间动脉和静脉中的表达也显著高于正常对照组(t=20.669,33.210,均P<0.05).VEGFR-2在肝硬化门脉高压组大鼠的小叶间静脉的表达显著高于小叶间动脉(t=23.424,P<0.05);而VEGFR-1在门脉高压组大鼠的小叶间动脉和小叶间静脉的表达几乎无明显差异(t=1.434,P>0.05).结论:VEGFR-2可能通过促进肝内小叶间静脉的新生血管生成代偿性降低门静脉高压.

AIM：To observe the distribution of vascular endothelial growth factor receptor-1 and-2 （VEGFR-1 and-2） in both hepatic arteries and veins of rats with portal hypertension （PHT）.METHODS：Thirty male Sprague-Dawley rats,weighing 180-220 g,were randomly divided into two groups：normal control group （n=10）,and model group （n=20）.The normal control group was given normal drinking water,while the model group was given drinking water containing thioacetamide （TAA）.Twelve weeks later,12 rats who had a portal pressure 1.57 kPa and obvious pseudolobules were selected for subsequent experiments.The distribution of VEGFR-1 and-2 in both hepatic arteries and veins of these rats was determined by immunohistochemistry.RESULTS：VEGFR-2 expression in both hepatic arteries and veins of PHT rats significantly increased （t=24.306,54.776,both P 0.05）.VEGFR-1 expression in both hepatic arteries and portal veins of PHT models also significantly increased （t=20.669,33.210,both P 0.05）.VEGFR-2 expression in hepatic veins was markedly higher than that in hepatic arteries in PHT rats （t=23.424,P 0.05）.There is no difference in VEGFR-1 expression between hepatic arteries and veins in PHT models （t=1.434,P 0.05）.CONCLUSION：VEGFR-2 can decrease the pressure of portal veins possibly by promoting the neovascularization of hepatic veins.