新疆汉族、维吾尔族及哈萨克族食管癌血清蛋白质指纹图谱

Identification of esophageal carcinoma-associated proteins by proteomics in Han,Uygur and Kazakh patients with esophageal carcinoma in Xinjiang,China

目的:探讨新疆主要民族之间食管癌患者血清蛋白质指纹图谱的差异.方法:用表面增强激光解吸电离飞行时间质谱技术(SELDI-TOF-MS)检测43例汉族、43例维吾尔族及41例哈萨克族食管癌血清蛋白质指纹图谱,所得结果用ZUCI-PDAS软件分析处理,筛选血清差异性蛋白质.结果:同民族间的血清蛋白质指纹图谱存在明显差异.汉族组与维吾尔族组中质荷比为4310.0109,8713.0142,7993.0223的3个蛋白质峰差异具有统计学意义(P<0.05);汉族组与哈萨克族食管癌组中质荷比为4310.0184,8167.9277,8158.1117,13789.4864,8067.7056,4611.9098,7993.4422,16146.8706的8个蛋白质峰差异具有统计学意义(P<0.05);维吾尔族组与哈萨克族组中质荷比分别为9161.7944,4611.6342,6649.6163,4979.3807的4个蛋白质峰差异具有统计学意义(P<0.05);质荷比4310.0109的蛋白峰在汉族组中呈低表达,而在维吾尔族组和哈萨克族组中均呈高表达.在维吾尔族组和哈萨克族组之间该蛋白峰未见到有差异.结论:汉族、维吾尔族及哈萨克族食管癌患者之间血清蛋白质指纹图谱的差异具有统计学意义,在新疆地区建立不同民族食管癌患者血清蛋白质指纹图谱疾病诊断模型有一定的临床意义.

AIM：To conduct a serum protein profile analysis in Han,Uygur and Kazakh patients with esophageal carcinoma （EC） in Xinjiang,China.METHODS：Serum samples from patients with EC （43 Han,43 Uygur and 41 Kazakh subjects） were detected by weak cation exchange （CM10） protein chip assay using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry （SELDI-TOF-MS） technology to screen differentially expressed serum markers for EC.RESULTS：The peaks at the mass to charge ratios （M/Z） 4 310.0109,8 713.0142 and 7 993.0223 were significantly different between Han and Uygur EC patients （P 0.05）.The peaks at M/Z 4 310.0184,8 167.9277,8 158.1117,13 789.4864,8 067.7056,4 611.9098,7 993.4422 and 16 146.8706 were significantly different between Han and Kazakh EC patients （P 0.05）.The peaks at M/Z 9 161.7944,4 611.6342,6 649.6163 and 4 979.3807 were significantly different between Uygur and Kazakh EC patients （P 0.05）.The peat at M/Z 4 310.0109 was highly expressed in Uygur and Kazakh patients but lowly expressed in Han patients.CONCLUSION：The protein fingerprints are significantly different among Han,Uygur and Kazakh EC patients in Xinjiang,China,which can be used to build a diagnostic model of EC.