大蝎子草水层部位对LPS诱导大鼠腹腔巨噬细胞iNOS与TNF-α的影响

Inhibited effects of Herba Girardinia water extract on expression of iNOS and TNF-α in macrophages induced by LPS

目的:研究大蝎子草水层部位对脂多糖(LPS)诱导大鼠腹腔巨噬细胞诱导型一氧化氮合酶(iNOS)表达、一氧化氮(NO)与肿瘤坏死因子α(TNF-α)分泌的影响。方法:建立体外培养大鼠腹腔巨噬细胞,以LPS为炎症刺激剂,免疫细胞化学研究大蝎子草水层部位不同剂量对LPS诱导大鼠腹腔巨噬细胞iNOS表达,Griess法分析培养液NO含量,间接MTT法分析培养液TNF-α含量。结果:LPS刺激大鼠腹腔巨噬细胞后,其iNOS表达显著增加,培养液中NO和TNF-α含量显著增加,大蝎子草水层部位对上述改变具有显著的抑制作用。结论:大蝎子草水层部位对细胞水平的炎症反应具有一定的抑制作用。

Objective：To investigate the inhibited effects of of Herba Girardinia water extract on inducible nitric oxide synthase （iNOS） expression in macrophages,and nitric oxide （NO） and tumor necrosis factor-alpha （TNF-α） excretion in macrophages induced by lipopolysaccharide （LPS）.Methods：The model was reproduced by LPS stimulating macrophages in culture in vitro.Induced NOS was detected by immuocytochemistry method,NO contents in medium were assayed by Griess method,and the TNF-α contents in medium were measured by indirect MTT method.Resutls：Induced NOS expression of macrophages,NO and TNF-α contents in medium of macrophages are significantly increased after the stimulation by LPS.Herba Girardinia water extract can remarkable ameliorate the indexes.Conclusion：Herba Girardinia water extract inhibits the inflammatory reaction at cell level.