淋巴细胞胞质蛋白在结肠直肠肿瘤中的表达及临床意义

Expression of L-plastin in plasma and tumor tissues of the patients with colorectal carcinoma and their clinical significance

目的:探讨淋巴细胞胞质蛋白(L-plastin)在结肠直肠肿瘤病人的血浆、肿瘤组织中的表达,并分析其与临床病理指标的关系。方法:采用ELISA法检测结肠直肠肿瘤病人与正常人血浆标本的L-plastin表达水平;并用免疫组化法定位检测,Western印迹法半定量测定组织中L-plastin的表达。结果:结肠直肠肿瘤病人血浆L-plastin的表达较正常人显著增高,肿瘤组织中L-plastin的表达亦较正常组织明显增高。而L-plastin高表达与肿瘤分化程度、肿瘤大小、淋巴转移等密切相关,与肿瘤侵犯深度和远处转移无关。结论:L-plastin在结肠直肠肿瘤病人的血浆、组织中表达显著增高,并与肿瘤分化程度、淋巴结转移有关,可能成为筛查、预后判断及基因靶向治疗的基础。

Objective To evaluate the expression of L-plastin in plasma and tumor tissues of patients with colorectal carcinoma,and to analyse their correlationship with the pathological staging and prognosis.Methods ELISA was used to detect L-plastin in plasma of 120 colorectal carcinoma patients and 40 normal persons;and Western blot was used to detect the expression of L-plastin in tissues.Results The positive rate of L-plastin expression both in plasma and in tissues was significantly higher in patients with colorectal carcinoma than in normal persons.The expression of L-plastin was significantly higher in the tumor tissues than in para-tumor tissues as shown by Western blot.L-plastin expression was correlated with tumor grading,the size of tumor and the degree of lymph node metastasis.But,the depth of tumor invasion and remote metastasis,bore no correlation with it.Conclusions The expression of L-plastin in plasma and tumor tissues of colorectal cancer patients is significantly increased,and is in correlation with the degree of tumor differentiation and lymph node metastasis.It might be used as the basis of screening,prognosis determination and targeted therapy in cases of colorectal carcinoma.