涎腺肿瘤中HSP90α、HSP90β和p53的表达及相关性

Expression and correlation of HSP90α,HSP90β and p53 in salivary tumors

目的探讨热休克蛋白90(HSP90)的两个亚型HSP90α和HSP90β及抑癌基因p53蛋白在涎腺良性、恶性肿瘤中的表达特征及相关性。方法采用免疫组化SP法检测41例良性和17例恶性肿瘤中HSP90α、HSP90β和p53的表达。结果 (1)HSP90α和HSP90β阳性以肿瘤上皮的胞浆表达为主,伴少量胞核表达;p53表达于肿瘤上皮的胞核,伴少量胞浆表达。(2)HSP90α和HSP90β在涎腺肿瘤中均明显表达,且表达强度正相关(P<0.01),尤其HSP90α在恶性肿瘤中的阳性表达率显著高于良性肿瘤(P<0.05)。(3)p53在肿瘤中高表达为突变型,p53在恶性肿瘤的表达显著高于良性肿瘤(P<0.05)。(4)在HSP90α和HSP90β阳性时,恶性肿瘤p53的表达率均显著高于良性肿瘤(P<0.01),而且p53阳性病例HSP90α的表达率在恶性肿瘤也显著高于良性肿瘤(P<0.05)。结论 (1)HSP90α和HSP90β、突变p53的持续刺激均是涎腺恶性肿瘤发生发展的基础。(2)HSP90α和HSP90β均可用作涎腺肿瘤治疗的重要的靶点。(3)HSP90α可能通过突变的p53蛋白促进肿瘤生长及恶性转化。

Objective To study the expression characteristics and correlation of the two-subtypes of heat shock protein 90（HSP90）（HSP90α,HSP90β） and tumor suppressor gene p53 in benign and malignant tumors of the salivary gland.Methods With immunohistochemistry SP techniques,the expressions of HSP90α,HSP90β and p53 were detected in 41 cases with benign tumors and 17 cases with malignant tumors.Results （1）The positive expressions of HSP90α and HSP90β were mainly in the cytoplasm with a small amount of nuclear expression.p53 was expressed in the nucleus of epithelial tumors with a small amount of cytoplasm.（2）In the salivary gland tumors,HSP90α and HSP90β were all significantly expressed,and the expression intensity was positively correlated each other（P0.01）.The expression rate of HSP90α in the malignant tumors was significantly higher than that in the benign tumors（P0.05）.（3）The expression of mutant p53 in tumors was high,and p53 expression in malignant tumors was significantly higher than that in benign tumors（P0.05）.（4） The expression rate of p53 in the malignant tumors was all significantly higher than that in the benign tumors in the cases with positive expressions of HSP90α and HSP90β（P0.01）,and HSP90α expression rate was higher in malignant tumors than that in the benign tumors in the cases with p53 positive expression（P0.05）.Conclusions （1） The continue stimulation of HSP90α,HSP90β and p53 is the foundation of the development of salivary gland malignant tumors.（2） HSP90α and HSP90β can be used as the targets for the treatment of salivary gland tumors.（3）HSP90α might promote tumor growth and malignant transformation through mutant p53.