黄酮对眼部血流量和脉络膜新生血管的作用(英文)

Effect of flavone on the ocular blood flow and formation of choroidal neovascularization

目的:观察黄酮对兔眼部血流量和大鼠脉络膜新生血管(CNV)的作用。方法:在体研究中,我们采用彩色微球技术测定黄酮对兔眼部血流量的影响。预先给予5g/L黄酮滴眼液(3次/d),1wk后用激光诱导大鼠CNV的生成,在2和4wk末,采用荧光血管造影(FA)测量CNV面积。离体研究中,采用MTT法检测黄酮对人脐静脉内皮细胞(HUVECs)存活率的影响。结果:黄酮显著的增加了兔眼部血流量,抑制了由激光诱导的CNV的生成。离体研究结果表明,黄酮抑制了HU-VEC的增生。结论:黄酮能够增加眼部血流量,并且抑制CNV的生成。

·AIM：To investigate the effect of flavone on ocular blood flow in rabbit eyes and the formation of choroidal neovascularization（CNV）in rat model of age-related macular degeneration（AMD）.·METHODS：In in vivo studies,colored microsphere technique was used to determine the ocular blood flow in ocular hypertensive rabbit eyes.The rat eyes were treated with 5g/L flavone eye drops 3 times a day for 1 week before and 4 weeks after laser-induced injury of Bruch＇s membrane.The development of CNV was determined by fluorescein angiography（FA）performed on weeks 2 and 4.In in vitro studies,the effect of flavone on the viability of HUVECs was measured by MTT assay.·RESULTS：The ocular blood flow in rabbit eyes was significantly increased after flavone instillation.Flavone significantly inhibited the formation of laser induced CNV.In vitro results showed that flavone inhibited the proliferation of HUVECs.·CONCLUSION：Flavone could increase ocular blood flow and inhibit the formation of CNV.·