EGFR反义寡核苷酸对人视网膜神经胶质细胞迁移的影响

Effect of EGFR antisense oligonucleotide on migration of retinal glial cells in vitro

目的:观察表皮生长因子受体(epidermal growth factor receptor,EGFR)反义寡核苷酸(antisense oligonucleotide,ASODN)对人视网膜神经胶质(retinal glial,RG)细胞迁移的影响。方法:人视网膜神经胶质细胞的原代培养,用脂质体(Li-pofectin)作为载体将修饰型EGFR ASODN转染RG细胞后,采用Murphy细胞计数方法评价反义寡核苷酸对人RPE细胞体外迁移的影响。结果:脂质体介导EGFR反义寡核苷酸组与对照组比较,细胞迁移活性受到明显抑制(P<0.05),转染24h后抑制率63.27%。结论:EGFR ASODN能够抑制人RG细胞迁移。

·AIM：To observe the effect of antisense oligonucleotide（ASODN）hybridized epidermal growth factor receptor（EGFR）on migration of human retinal glial（RG）cells.·METHODS：Human RG cells were cultured in vitro.Modified EGFR ASODN was transfected into RG cells by lipofectin.By using an in vitro wound healing model in which a small area of a confluent monolayer of RPE cells was denuded,migration was measured by the number of cells that had entered the denuded area.·RESULTS：The cell migration was inhibited effectively by ASODN with lipofectin at 63.27%.The missense oligonucleotides showed no such effect（P〈0.01）.·CONCLUSION：EGFR ASODN can inhibit the migration of RG cells.·