糖痹康对大鼠糖尿病周围神经病变氧化应激影响的实验研究

The influences of Tangbikang on oxidative stress in rats with diabetic peripheral neuropathy

目的 探讨糖痹康对链脲佐菌素（Streptozotocin,STZ）诱导的糖尿病周围神经病变（Diabetic penipheral neuropathy,DPN）大鼠抗氧化作用的影响.方法 腹腔注射STZ 55 mg/kg建立糖尿病大鼠模型,分为正常组,模型组,弥可保组,糖痹康低、中、高剂量组.各组每周测1次体重,8周后处死大鼠,测定血清中血糖（Glu）、超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮合成酶（NOS）、一氧化氮（NO）和糖基化终产物（AGEs）含量及坐骨神经中醛糖还原酶（AR）和Na＋-K＋-ATP酶活性.结果 坐骨神经组织病理学检查显示,糖痹康组坐骨神经病理学改变较模型组轻.与模型组比较,糖痹康各剂量组大鼠体重第8周差异有统计学意义（P均＜0.01）;血清SOD、NOS和NO含量增高（P均＜0.05）,AGEs、MDA含量显著降低（P均＜0.01）;坐骨神经AR浓度显著降低（P均＜0.01）,Na＋-K＋-ATP酶表达显著增加（P均＜0.05）.结论 糖痹康可改善STZ诱导的DPN过高的氧化应激状态,减轻氧化应激造成的周围神经结构和功能损害,对DPN有一定的神经保护作用.

Objective To investigate the influences of Tangbikang on the oxidative stress in rats with diabetic penipheral neuropathy （DPN）. Methods Diabetes rats were induced by intraperitoneal injection of STZ, 55 mg/kg and randomly divided into five groups： model group, methycobal treatment group, and Tang Bi Kang low, medium and high dosage treatment group. Body weight was examined every week. All rats were sacrificed at week 8 and the values of blood serum, superoxide dismutase, malondialdehyde, nitric oxide synthase and nitric oxide were detected. Results After treated with Tang Bi Kang, the eighth week＇s body weight, the levels of SOD, NOS and NO in the blood serum and ATP of sciatic nerve were increased greatly （P＜0.01 or P＜0.05） and the contents of MDA and AGEs and AR of sciatic nerve were decreased （P＜0.01）, compared with the model group. Conclusion Tang Bi Kang may lighten the oxidative stress reaction of diabetic penipheral neuropathy and slow down the course of the diabetic penipheral neuropathy.