水分胁迫对寒富苹果叶片碳氮代谢的影响

Effects of Water Stress on Carbon and Nitrogen Metabolism of Hanfu Apple Leaves

以盆栽"寒富"苹果为试材,研究了不同水分胁迫处理对"寒富"苹果碳氮代谢的影响。结果表明:水分胁迫使"寒富"苹果叶片内可溶性糖、游离氨基酸和可溶性蛋白含量升高,淀粉含量降低,胁迫越严重,变化幅度越大。轻度干旱胁迫提高了"寒富"苹果叶片中性转化酶、酸性转化酶和蔗糖合成酶活性,降低了蔗糖磷酸合成酶、山梨醇脱氢酶、山梨醇氧化酶、硝酸还原酶、亚硝酸还原酶、谷丙转氨酶、谷草转氨酶和谷氨酰胺合成酶的活性;中度、重度干旱胁迫和淹水条件下,中性转化酶、酸性转化酶和蔗糖合成酶降低,蛋白酶活性增加。"寒富"苹果能忍耐轻度和中度干旱胁迫,抗旱性较强。

Using potted Hanfu apple as materials, carbon and nitrogen substance metabolism of Hanfu apple under different water stress was studied. The results showed that soluble sugar, free amino acids and soluble protein content increased while starch content reduced under water stress conditions. The heavier of stress, the greater of change range. Under light drought stress, neutral invertase, acid invertase and sucrose synthase activity improved, sucrose phosphate synthase, sorbitol dehydrogenase, sorbitol oxidase, nitrate reductase, nitrite reductase, glutamic-pyruyic transaminase, glutamic-oxaloacetic transaminase and glutamine synthetase activity reduced. In moderate and high drought stress and waterlogging conditions, neutral invertase, acid invertase and sucrose synthase activity reduced and protease activity enhanced. Hanfu apple was a cuhivar with strong drought resistance because it could tolerate light and moderate drought.