硬皮病皮损成纤维细胞整合素表达的免疫电镜观察

Study on expression of integrins in fibroblasts in patients with scleroderma by immunoelectron microscope

目的：研究硬皮病皮损成纤维细胞整合素表达及细胞超微结构特征。方法：将培养的硬皮病皮损成纤维细胞置包被纤维粘连蛋白的培养瓶中作粘附培养，运用间接免疫胶体金技术在电镜下观察成纤维细胞表面整合素α５，β１亚基表达、分布特征及细胞超微结构变化。结果：硬皮病皮损成纤维细胞整合素α５，β１亚基表达均较正常成纤维细胞显著增加（Ｐ＜０．０１），并呈簇集分布的特征；细胞内骨架成分微丝密集成束。结论：整合素α５，β１介导的纤维粘连蛋白与成纤维细胞相互作用在硬皮病时增强。

Objective: To study the expression of integrins in fibroblasts in patients with scleroderma. Methods: Employing technique of indirect immunocolloidal gold and electron microscope, we observed the expression of integrin α 5 ,β 1 subunits and the supermicro structures of sclerderma fibroblasts cultured in medium absorbed with fibronectin. Results: The results showed that the expression of integrin α 5 ,β 1 subunits in fibroblasts from scleroderma was higher than that in normal fibroblasts ( P <0.01), and colloidal golds clustered on the surfaces of scleroderma fibroblasts. The cytoskeletal structure: microfilaments were dense and aggregated into bundles. Conclusion: All of these results suggest that the interactions of fibroblasts with fibronectin mediated by integrin α 5 ,β 1 subunits increase in scleroderma.