种属特异性PCR技术在检测鹅肥肝掺假中的应用研究

Detection of fraudulent substitution of the duck liver for goose fat liver using species-specific polymerase chain reaction

建立起一种用于检测鹅肥肝是否掺假的方法:种属特异性PCR技术。以鹅、鸭的12SrRNA基因序列设计特异引物,扩增目的片段分别为97bp和196bp。以三类温度处理和0.1%～100%的鸭肥肝掺假比例,分析方法的有效性和灵敏性。结果表明,这种方法的效果是不受样品被长时间加热的影响(高至100℃,10min);同时,即使当掺假比例只有0.1%时,仍能有效扩增出2特异条带。说明本方法适用于快速、准确地检测鹅肥肝掺假的需要。

A specific Polymerase Ch ain Reaction has been developed for the detection of fraudulent substitution of the duck liver for goose liver. The primers were designed to be specific for goose and duck, targeting the 12S rRNA mitochondrial gene. Analysis of experimental mixtures demonstrated that the detection limit of the assay was approximately 0.1% for fraudulent substitution, and two specific amplification bands were still showed in this case. The performance of this method was not affected by prolonged heat-treatment （up. to 100℃,10 min at 100 kPa ）. Consequently,this technology can be very useful for rapidly and accuratly detecting fraudulent substitution of the duck liver for the more expensive goose fat liver.